Bleaching characteristics of rhodopsin from normal and dystrophic rats
| Autor: | Theodore P. Williams, Michael H. Chaitin |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Thermal denaturation
Orange light Rhodopsin genetic structures Retinal dystrophy In Vitro Techniques Biology Metarhodopsin II Retina Cellular and Molecular Neuroscience chemistry.chemical_compound Pigment Animals Photolysis Retinal Degeneration Temperature Sensory Systems Rats Ophthalmology Digitonin chemistry Biochemistry visual_art biology.protein Biophysics visual_art.visual_art_medium sense organs Retinal Pigments |
| Zdroj: | Experimental Eye Research. 24:553-558 |
| ISSN: | 0014-4835 |
| Popis: | Rhodopsin from the retinas of dystrophic (RCS) and normal rats was extracted in 2% digitonin. With the bleaching of visual pigment samples by short exposures to orange light it is shown that no difference exists for the bleaching rates of the two rhodopsins at 22 days of age. This finding is contrary to the suggestion that dystrophic rat retinas contain a form of rhodopsin which is unusually labile to the action of light. The thermal stability of the rhodopsin from 22 day old dystrophic rats was compared to that of adult normal rats at a temperature of 52°C. Again, no difference was observed indicating that the rate limiting step for thermal denaturation is normal in dystrophic rat rhodopsin. The rate of formation of metarhodopsin II was studied for rhodopsin from 25-day-old dystrophic and normal rats. At the three temperatures in which it was tested, 17, 25 and 37°C, the formation of this photoproduct appears to be normal for the dystrophic rat rhodopsin. |
| Databáze: | OpenAIRE |
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