Erythromycin-resistant Streptococcus pneumoniae: phenotypes, genotypes, transposons and pneumococcal vaccine coverage rates
| Autor: | Nezahat Gürler, Seyyide Sariçam, Gülşen Hasçelik, K. S. Diker, İnci Başak Müştak, Fatma Nur Akdogan Kittana |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Genotype Turkey Tetracycline 030106 microbiology Drug resistance Biology Serogroup medicine.disease_cause Microbiology Pneumococcal Infections Pneumococcal conjugate vaccine Pneumococcal Vaccines 03 medical and health sciences Antibiotic resistance Drug Resistance Multiple Bacterial Streptococcus pneumoniae medicine Humans Bacterial Capsules General Medicine Pneumococcal polysaccharide vaccine Anti-Bacterial Agents Erythromycin Phenotype 030104 developmental biology Pneumococcal vaccine DNA Transposable Elements Quellung reaction medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 68:874-881 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Purpose. To assess the antibiotic resistance, transposon profiles, serotype distribution and vaccine coverage rates in 110 erythromycin-resistant S. pneumoniae clinical isolates. Methodology. Erythromycin, clindamycin, tetracycline, chloramphenicol and kanamycin susceptibilities were assessed using the E-test/disc diffusion method. Inducible macrolide resistance was tested using the erythromycin-clindamycin double disc diffusion test. Serogrouping and serotyping were performed using latex particle agglutination and the Quellung reaction, respectively. Drug resistance genes and transposon-specific genes were investigated by PCR. Results. Of the isolates, 93 % were resistant to clindamycin; 81 % were resistant to tetracycline; 76 % were multi-drug-resistant, having resistance to both clindamycin and tetracycline; and 12 % had extended-drug resistance, being resistant to clindamycin, tetracycline, chloramphenicol and kanamycin. The majority of isolates (88.2 %) exhibited the cMLSB phenotype. The association between the cMLSB phenotype and tetracycline resistance was related to transposons Tn2010 (38.2 %), Tn6002 (21.8 %) and Tn3872 (18.2 %). M and iMLSB phenotypes were observed in 7 and 5 % of the isolates, respectively. The most frequent serotype was 19 F (40 %). Among the erythromycin-resistant pneumococci, vaccine coverage rates for the 13-valent pneumococcal conjugate vaccine (PCV-13) and the 23-valent pneumococcal polysaccharide vaccine (PPSV-23) were 76.4 and 79.1 %, respectively, compared to 82.2 and 85.1 % transposon-carrying isolates. Conclusions. Multi-drug resistance among erythromycin-resistant S. pneumoniae isolates mainly occurs due to the horizontal spread of the Tn916 family of transposons. The majority of the transposon-carrying isolates are covered by 13- and 23-valent pneumococcal vaccines. Since serotype distribution and transposons in S. pneumoniae isolates may change over time, close monitoring is essential. |
| Databáze: | OpenAIRE |
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