Molecular epidemiology and genetic diversity of human rhinovirus affecting hospitalized children in Rome

Autor: Eleonora Cella, Carolina Scagnolari, Guido Antonelli, Stefano Chiavelli, Massimo Ciccozzi, Maurizio Muraca, Marta Giovanetti, Paola Papoff, Corrado Moretti, Alessandra Pierangeli, Fabio Midulla, Carlo Concato, Lucia Spano
Přispěvatelé: Department of Molecular Medicine, Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Department of Infectious Diseases, Istituto Superiore di Sanita [Rome], Research Institute, IRCCS Ospedale Pediatrico Bambino Gesù [Roma], Paedriatic Department, Umberto I Hospital, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], This study was supported by grants from Pasteur Institute, Cenci Bolognetti Foundation to G.A and from Sapienza University of Rome ‘‘Ricerche Universitarie’’ to CS.
Rok vydání: 2013
Předmět:
Male
MESH: Sequence Analysis
DNA
viruses
Rome
phylogeny
medicine.disease_cause
Medical microbiology
MESH: Picornaviridae Infections
Genotype
Prevalence
Cluster Analysis
Immunology and Allergy
MESH: Genetic Variation
capsid protein
rhinovirus
genotyping
MESH: Phylogeny
Molecular Epidemiology
0303 health sciences
MESH: Rhinovirus
Phylogenetic tree
virus diseases
General Medicine
MESH: Infant
3. Good health
MESH: 5' Untranslated Regions
Female
Rhinovirus
circulatory and respiratory physiology
Microbiology (medical)
medicine.medical_specialty
Molecular Sequence Data
Immunology
MESH: Child
Hospitalized
MESH: Viral Structural Proteins
Biology
03 medical and health sciences
stomatognathic system
otorhinolaryngologic diseases
medicine
Humans
MESH: Molecular Epidemiology
Gene
Genotyping
MESH: Prevalence
Retrospective Studies
030304 developmental biology
Viral Structural Proteins
Genetic diversity
Picornaviridae Infections
MESH: Humans
MESH: Molecular Sequence Data
Molecular epidemiology
030306 microbiology
Genetic Variation
Infant
MESH: Retrospective Studies
Sequence Analysis
DNA
MESH: Cluster Analysis
Virology
MESH: Male
MESH: Rome
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
5' Untranslated Regions
Child
Hospitalized
MESH: Female
Zdroj: Medical Microbiology and Immunology
Medical Microbiology and Immunology, Springer Verlag, 2013, 202 (4), pp.303-11. ⟨10.1007/s00430-013-0296-z⟩
ISSN: 1432-1831
0300-8584
DOI: 10.1007/s00430-013-0296-z
Popis: International audience; Human rhinoviruses (HRV) have been re-classified into three species (A-C), but the recently discovered HRV-C strains are not fully characterized yet. This study aimed to undertake a molecular and epidemiological characterization of HRV strains infecting children hospitalized over one year in two large research hospitals in Rome. Nasal washings from single HRV infections were retrospectively subjected to phylogenetic analysis on two genomic regions: the central part of the 5'Untranslated Region (5'UTR) and the Viral Protein (VP) 4 gene with the 5' portion of the VP2 gene (VP4/2). Forty-five different strains were identified in 73 HRV-positive children: 55 % of the cases were HRV-A, 38 % HRV-C and only 7 % HRV-B. HRV-C cases were less frequent than HRV-A during summer months and more frequent in cases presenting wheezing with respect to HRV-A. Species distribution was similar with respect to patient age, and seasonality differed during summer months with fewer HRV-C than HRV-A cases. On admission, a significantly higher number of HRV-C cases presented with wheezing with respect to HRV-A. The inter- and intra-genotype variability in VP4/2 was higher than in 5'UTR; in particular, HRV-A patient VP4/2 sequences were highly divergent (8-14 %) at the nucleotide level from those of their reference strains, but VP4 amino acid sequence was highly conserved. In HRV-C isolates, the region preceding the initiator AUG, the amino acids involved in VP4 myristoylation, the VP4-VP2 cleavage site and the cis-acting replication element were highly conserved. Differently, VP4 amino acid conservation was significantly lower in HRV-C than in HRV-A strains, especially in the transiently exposed VP4 N-terminus. This study confirmed the high number of different HRV genotypes infecting hospitalized children over one year and reveals a greater than expected variability in HRV-C VP4 protein, potentially suggestive of differences in replication.
Databáze: OpenAIRE
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