A Multifunctional Neutralizing Antibody‐Conjugated Nanoparticle Inhibits and Inactivates SARS‐CoV‐2

Autor: John J. Fung, Aleksander Prominski, Jillian Rosenberg, Dominique Missiakas, Nicholas Ankenbruck, Eugene B. Chang, Min Chen, Mindy Nguyen, Jun Huang, Pablo Penaloza-MacMaster, Glenn Randall, Xiaolei Cai, Anastasia Tomatsidou, Jiuyun Shi, Bozhi Tian, Yiliang Lin
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Hot Temperature
Immunoconjugates
Light
Polymers
General Chemical Engineering
viruses
Drug Evaluation
Preclinical
General Physics and Astronomy
Medicine (miscellaneous)
medicine.disease_cause
Antibodies
Viral
SARS‐CoV‐2
Polyethylene Glycols
Antigen-Antibody Reactions
chemistry.chemical_compound
Mice
General Materials Science
Neutralizing antibody
Research Articles
Coronavirus
biology
Chemistry
General Engineering
Spike Glycoprotein
Coronavirus
virus inactivation
Receptors
Virus
Angiotensin-Converting Enzyme 2
Antibody
Research Article
photothermal therapy
Science
Polyethylene glycol
Biochemistry
Genetics and Molecular Biology (miscellaneous)
Virus
multifunctional nanoparticle
In vivo
COVID‐19
Thiadiazoles
medicine
Animals
Humans
SARS-CoV-2
Phosphatidylethanolamines
fungi
COVID-19
Photothermal therapy
Virology
Antibodies
Neutralizing
In vitro
Semiconductors
biology.protein
Nanoparticles
Zdroj: Advanced Science, Vol 9, Iss 2, Pp n/a-n/a (2022)
Advanced Science
ISSN: 2198-3844
Popis: The outbreak of 2019 coronavirus disease (COVID‐19), caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), has resulted in a global pandemic. Despite intensive research, the current treatment options show limited curative efficacies. Here the authors report a strategy incorporating neutralizing antibodies conjugated to the surface of a photothermal nanoparticle (NP) to capture and inactivate SARS‐CoV‐2. The NP is comprised of a semiconducting polymer core and a biocompatible polyethylene glycol surface decorated with high‐affinity neutralizing antibodies. The multifunctional NP efficiently captures SARS‐CoV‐2 pseudovirions and completely blocks viral infection to host cells in vitro through the surface neutralizing antibodies. In addition to virus capture and blocking function, the NP also possesses photothermal function to generate heat following irradiation for inactivation of virus. Importantly, the NPs described herein significantly outperform neutralizing antibodies at treating authentic SARS‐CoV‐2 infection in vivo. This multifunctional NP provides a flexible platform that can be readily adapted to other SARS‐CoV‐2 antibodies and extended to novel therapeutic proteins, thus it is expected to provide a broad range of protection against original SARS‐CoV‐2 and its variants.
A multifunctional neutralizing antibody‐conjugated nanoparticle is developed to capture and inactivate SARS‐CoV‐2. It efficiently captures SARS‐CoV‐2 pseudovirions and completely blocks viral infection of host cells in vitro through the surface neutralizing antibodies. In addition, its photothermal function inactivates viruses upon light irradiation. More importantly, it treats authentic SARS‐CoV‐2 infection in vivo outperforming neutralizing antibodies.
Databáze: OpenAIRE
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