Differential ex vivo nitric oxide production by acutely isolated neonatal and adult microglia
| Autor: | Margo R. Roberts, Courtney A. Crane, Michael F. Smith, John B. Schell |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Lipopolysaccharides
Chemokine Interferon Inducers medicine.medical_treatment Immunology Central nervous system Nitric Oxide Synthase Type II Enzyme-Linked Immunosorbent Assay Biology Nitric Oxide Article Nitric oxide Rats Sprague-Dawley Interferon-gamma Lipopeptides Mice chemistry.chemical_compound medicine Animals Immunology and Allergy Drug Interactions Interferon gamma Cells Cultured Interferon inducer Microglia Reverse Transcriptase Polymerase Chain Reaction Toll-Like Receptors Gene Expression Regulation Developmental Rats Mice Inbred C57BL Poly I-C medicine.anatomical_structure Cytokine nervous system Neurology chemistry biology.protein Cytokines Neurology (clinical) Peptides Ex vivo medicine.drug |
| Zdroj: | Journal of Neuroimmunology. 189:75-87 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/j.jneuroim.2007.07.004 |
| Popis: | Microglia are the macrophage population residing in the parenchyma of the central nervous system (CNS), and are thought to play critical roles in CNS development, homeostasis and defense against pathogens. Microglia are capable of rapidly responding to microbial pathogens through engagement of their Toll-like receptors (TLRs). We first compared the efficiency of these responses in primary microglia acutely isolated from adult and neonatal mice. While the cytokine and chemokine responses of adult microglia were generally higher than those of neonatal cells stimulated ex vivo through TLRs, the nitric oxide response of neonatal microglia was markedly enhanced relative to the adult cells. We then went on to identify culture conditions such as exposure to M-SCF or GM-CSF that markedly enhanced the nitric oxide response of microglia, particularly those from the adult CNS. Finally, we demonstrate that the differential nitric oxide response of neonatal and adult microglia is not only limited to the mouse, but also extends to rat microglia. |
| Databáze: | OpenAIRE |
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