Identification of Molecular Determinants of Resistance to Pyrethroid Insecticides in Aedes aegypti (Diptera: Culicidae) Populations in California, USA
| Autor: | Kelly A Liebman, Sarah A. Billeter, Robert Payne, Melissa Hardstone Yoshimizu, Renjie Hu, Marco E. Metzger, Kerry A. Padgett, Mary Joyce Pakingan, Vicki L. Kramer, Fan Yang, Samuel Schildhauer |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Insecticides Pesticide resistance Genotype knockdown resistance 030231 tropical medicine Population Zoology Mosquito Vectors Aedes aegypti Biology medicine.disease_cause 01 natural sciences California Zika virus Insecticide Resistance 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Aedes Pyrethrins parasitic diseases Vector Control Pest Management Resistance Repellents medicine Animals Chikungunya education education.field_of_study Pyrethroid General Veterinary Knockdown resistance Pesticide biology.organism_classification 3. Good health 010602 entomology Infectious Diseases chemistry Insect Science Parasitology |
| Zdroj: | Journal of Medical Entomology |
| ISSN: | 1938-2928 0022-2585 |
| DOI: | 10.1093/jme/tjz076 |
| Popis: | The first breeding populations of Aedes aegypti (Linnaeus) were identified in California in 2013, and have since been detected in 13 counties. Recent studies suggest two introductions likely occurred, with genetically distinct populations in the central and southern regions of the state. Given the threat of dengue, chikungunya, and Zika virus transmission, it is imperative to understand if these populations harbor genes that could confer resistance to pyrethrin-based insecticides, known as pyrethroids, the most commonly used class of adulticides in the state. In 2017, the California Department of Public Health initiated a pesticide resistance screening program for Ae. aegypti to assess the presence of specific mutations on the sodium channel gene (V1016I and F1534C) associated with knockdown resistance to pyrethroids. Mosquitoes collected between 2015 and 2017 from 11 counties were screened for mutations using real-time polymerase chain reaction assays. Results revealed distinctly different resistance profiles between the central and southern regions. The central population displayed nearly fixed resistant mutations at both loci, whereas the southern population was more variable. The relative proportion of resistant alleles observed in sampled mosquitoes collected in southern California increased each year from 2015 through 2017, indicating potential increases in resistance across this region. The presence of these mutations indicates that these mosquitoes may be predisposed to surviving pyrethroid treatments. Additional biological and biochemical assays will help better elucidate the mechanisms underlying insecticide resistance in California Ae. aegypti and prompt the use of pesticides that are most effective at controlling these mosquitoes. |
| Databáze: | OpenAIRE |
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