Development of Injectable Thermosensitive Chitosan-Based Hydrogels for Cell Encapsulation
| Autor: | Alessandro Romano, Francesca Gervaso, Velia La Pesa, Lorenzo Moroni, Antonella Stanzione, Giuseppe Gigli, Alessandro Polini, Angelo Quattrini |
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| Přispěvatelé: | CTR, RS: MERLN - Complex Tissue Regeneration (CTR) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scaffold
in vitro degradation injectable hydrogel 02 engineering and technology 01 natural sciences lcsh:Technology Chitosan Extracellular matrix lcsh:Chemistry chemistry.chemical_compound natural polymers General Materials Science Cell encapsulation Instrumentation lcsh:QH301-705.5 Fluid Flow and Transfer Processes L-ARGININE SCAFFOLD General Engineering Adhesion 021001 nanoscience & nanotechnology lcsh:QC1-999 Computer Science Applications Self-healing hydrogels Swelling medicine.symptom 0210 nano-technology MATRICES Biocompatibility 010402 general chemistry cell encapsulation biocompatibility medicine swelling ratio GLYCEROPHOSPHATE 3D matrices lcsh:T Process Chemistry and Technology technology industry and agriculture IN-VITRO IMPLANTS GELATION 0104 chemical sciences chemistry Chemical engineering lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 CULTURE-SYSTEMS stimuli-responsive hydrogel lcsh:Engineering (General). Civil engineering (General) lcsh:Physics |
| Zdroj: | Applied sciences-Basel, 10(18):6550. Multidisciplinary Digital Publishing Institute (MDPI) Applied Sciences, Vol 10, Iss 6550, p 6550 (2020) Applied Sciences Volume 10 Issue 18 |
| Popis: | The three-dimensional complexity of the native extracellular matrix (ECM) suggests switching from 2D to 3D culture systems for providing the cells with an architecture more similar to the physiological environment. Reproducing the three-dimensionality in vitro can guarantee beneficial effects in terms of cell growth, adhesion, proliferation, and/or their differentiation. Hydrogels have the same tailorable physico-chemical and biological characteristics as ECM materials. In this study, we propose a thermoresponsive chitosan-based hydrogel that gels thanks to the addition of organic and inorganic salt solutions (beta-glycerolphosphate and sodium hydrogen carbonate) and is suitable for cell encapsulation allowing obtaining 3D culture systems. Physico-chemical analyses showed that the hydrogel formulations jellify at physiological conditions (37 ° C, pH 7.4), are stable in vitro up to three weeks, have high swelling ratios and mechanical stiffness suitable for cellular encapsulation. Moreover, preliminary biological tests underlined the pronounced biocompatibility of the system. Therefore, these chitosan-based hydrogels are proposed as valid biomaterials for cell encapsulation. |
| Databáze: | OpenAIRE |
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