A comparative transcriptomic analysis of replicating and dormant liver stages of the relapsing malaria parasite Plasmodium cynomolgi
| Autor: | Clemens H. M. Kocken, Judith Knehr, Devendra Kumar Gupta, Ghislain M. C. Bonamy, Bart W. Faber, Boon Heng Lee, Sam O. Hofman, Erica M. Pasini, Sven Schuierer, Florian Nigsch, Tewis Bouwmeester, Walter Carbone, Annemarie Voorberg-van der Wel, Thierry T. Diagana, Guglielmo Roma, Bernd Kinzel, Anne-Marie Zeeman, Pablo Bifani |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genome integrity QH301-705.5 Science malaria Biology General Biochemistry Genetics and Molecular Biology Transcriptome 03 medical and health sciences transcriptomics Plasmodium cynomolgi parasitic diseases medicine Parasite hosting Biology (General) liver stages General Immunology and Microbiology Drug discovery General Neuroscience General Medicine medicine.disease Virology 3. Good health Gene expression profiling 030104 developmental biology hypnozoites plasmodium Infectious disease (medical specialty) Medicine Malaria |
| Zdroj: | eLife, Vol 6 (2017) |
| Popis: | Plasmodium liver hypnozoites, which cause disease relapse, are widely considered to be the last barrier towards malaria eradication. The biology of this quiescent form of the parasite is poorly understood which hinders drug discovery. We report a comparative transcriptomic dataset of replicating liver schizonts and dormant hypnozoites of the relapsing parasite Plasmodium cynomolgi. Hypnozoites express only 34% of Plasmodium physiological pathways, while 91% are expressed in replicating schizonts. Few known malaria drug targets are expressed in quiescent parasites, but pathways involved in microbial dormancy, maintenance of genome integrity and ATP homeostasis were robustly expressed. Several transcripts encoding heavy metal transporters were expressed in hypnozoites and the copper chelator neocuproine was cidal to all liver stage parasites. This transcriptomic dataset is a valuable resource for the discovery of vaccines and effective treatments to combat vivax malaria. |
| Databáze: | OpenAIRE |
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