Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus
| Autor: | Jungho Kim, Daesub Song, Kwang-Soo Lyoo, Woonsung Na, Gun-Woo Ha, Donghyuk Kim, Minjoo Yeom, Van Phan Le |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Paper Time Factors Swine Prevalence Sensitivity and Specificity Chromatography Affinity 03 medical and health sciences Feces Porcine epidemic diarrhoea virus Watery diarrhoea Medicine Animals Pig farms rapid diagnosis Reagent Strips Swine Diseases Strip test General Veterinary biology business.industry Porcine epidemic diarrhea virus immunochromatographic test kit General Medicine biology.organism_classification Virology 030104 developmental biology Epidemiological surveillance business Coronavirus Infections |
| Zdroj: | The Veterinary Record Veterinary Record |
| ISSN: | 2042-7670 0042-4900 |
| Popis: | Porcine epidemic diarrhoea virus (PEDV) causes acute and severe watery diarrhoea and dehydration, as well as 50–100 per cent mortality in piglets. For the PEDV diagnosis, a rapid test kit that is specific and sensitive to PEDV is critical to monitor this disease at pig farms. The present study aimed to develop an immunochromatographic assay (ICA) strip test for detecting PEDV in faecal swabs. The newly developed diagnostic test showed a detection limit of 10 4.0 TCID 50 /ml of PEDV. Using faecal swab samples, the relative sensitivity and specificity of the ICA kit were 95.0 per cent and 98.6 per cent, respectively, compared with those of real-time RT-PCR. In samples from piglets experimentally infected with PEDV, the results showed 100 per cent agreement with those found by real-time RT-PCR. Our developed test strip will be useful for rapid diagnosis and can be used for epidemiological surveillance of PEDV infection. |
| Databáze: | OpenAIRE |
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