Effect of sperm dosage transportation in stallions: Effect on sperm DNA fragmentation

Autor: F. Arroyo, J C de la Torre, A.S. Abdoon, Jaime Gosálvez, Mario Zabal-Aguirre, F. Crespo
Přispěvatelé: Ministerio de Asuntos Exteriores (España), Ministerio de Economía y Competitividad (España), Torre, J. de la, Torre, J. de la [0000-0002-2105-5655]
Rok vydání: 2018
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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ISSN: 1873-2232
Popis: Artificial insemination programs for horses usually involve ex vivo handling and transporting of sperm. The present experiment was designed to: (i) assess the effect of transportation on sperm DNA integrity at different time post semen collection, and (ii) evaluate if sperm DNA quality deteriorates rapidly beyond 24 h of cooled storage. After collection, the ejaculates were extended using INRA 96 and semen was prepared for prompt analysis (A0) or 24 h/48 h cooled-shipping (B24 and C48 respectively). Each sample was assessed for sperm DNA fragmentation index (SDFI) at time 0 and after incubation for 2, 6 and 24 h at 37 °C. There was very little difference in SDFI between freshly extended (A0) and 24 h/48 h cooled-transported semen samples (B24/C48) at time 0. After 2 h of incubation at 37 °C, there was an increase in SDFI ranging from 2.7% to 7.5% per hour in freshly extended semen samples (A0: 5.1 ± 1.5), while cooled-transported semen samples had a much greater increase in SDFI, ranging from 5.0% to 20.5% (B24: 14.7 ± 5.6) and from 8.2% to 26.8% (C48: 18.3 ± 7.2) respectively. There were not marked differences in the sperm DNA integrity between 24 and 48 h for transported samples, thus there is the possibility of desirable fertility with use of stallion sperm after 48 h of cooled storage.
This research was supported with public funding from the Spanish Agency for International Cooperation and Development (AECID: AP/039620/11) and the Spanish Ministry of Economy and Competitiveness (MINECO: BFU-2013-44290-R).
Databáze: OpenAIRE
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