Analysis of corneal stromal roughness after iFS 150 kHz and LenSx femtosecond LASIK flap creation in porcine eyes
| Autor: | Rafael Cañones-Zafra, Miguel A. Teus, Alberto Parafita-Fernández, Santiago Casado, Samira Ketabi, Juan Gros-Otero, Montserrat Garcia-Gonzalez, César Villa-Collar |
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| Rok vydání: | 2019 |
| Předmět: |
Stromal cell
Materials science genetic structures Swine Corneal Stroma medicine.medical_treatment Keratomileusis Laser In Situ Visual Acuity Keratomileusis Surface finish Microscopy Atomic Force Surgical Flaps law.invention 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cirugía law Myopia medicine Surface roughness Animals Postoperative Period Atomic force microscopy LASIK Cirugía láser Laser eye diseases Sensory Systems Disease Models Animal Ophthalmology Femtosecond 030221 ophthalmology & optometry Oftalmología Lasers Excimer sense organs 030217 neurology & neurosurgery Rayo láser Biomedical engineering |
| Zdroj: | Repositorio Institucional del Instituto Madrileño de Estudios Avanzados en Nanociencia instname |
| ISSN: | 1435-702X 0721-832X |
| DOI: | 10.1007/s00417-019-04497-7 |
| Popis: | Purpose To describe and compare the stromal bed roughness obtained after laser in situ keratomileusis (LASIK) flap creation using a corneal femtosecond laser platform (iFS 150) and a “dual” femtosecond (FS) laser platform (LenSx). Methods This ex vivo experimental study was conducted in an animal model using 12 freshly enucleated porcine eyes, six with each femtosecond laser. The standard laser treatment parameters were used for the experiment. After LASIK flap creation, the corneal stromal roughness was assessed using atomic force microscopy (AFM) in contact mode immersed in liquid. In each sample, surface measurements were obtained in 60 regions of six eyes per FS laser in 10 20 × 20-micron areas of the central corneal stroma at 512 × 512-point resolution. The surface roughness was measured and the root-mean-square (RMS) values of the roughness were obtained. Results The mean RMS ± standard deviation values were 430 ± 150 nm for the corneal femtosecond laser platform and 370 ± 100 nm for the dual FS laser platform (P < 0.011). Conclusions In this experimental study with AFM, we found smoother stromal beds after LASIK flap creation with LenSx compared to iFS 150 kHz. Further studies are needed to understand visual implications of the differences found. Sin financiación No data JCR 2019 1.265 SJR (2019) Q1, 16/124 Ophthalmology; Q2, 11/43 Sensory Systems; Q3, 45/89 Cellular and Molecular Neuroscience No data IDR 2019 UEM |
| Databáze: | OpenAIRE |
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