Contaminated water confirmed as source of infection by bioassay in an outbreak of toxoplasmosis in South Brazil
| Autor: | Camila Ribeiro Silva, Luís Antônio Sangioni, Camila Encarnação Minuzzi, Patricia Bräunig, Paulo Dilkin, Carlos Augusto Mallmann, Jessica dos Santos Ribeiro, Fagner D'ambroso Fernandes, Diego Artemio Franco Sturza, Ivone Andreatta Menegolla, Leandro Giacomini, Emerson Salvagni, Lourdes Bonfleur Farinha, Cledison Marcio Difante, Fernanda Silveira Flores Vogel, Luiza Pires Portella, Gisele Gehrke |
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| Rok vydání: | 2020 |
| Předmět: |
Veterinary medicine
Swine 040301 veterinary sciences animal diseases Biology Disease Outbreaks Serology law.invention 0403 veterinary science 03 medical and health sciences law parasitic diseases medicine Animals Humans Ingestion Bioassay Polymerase chain reaction 030304 developmental biology 0303 health sciences General Veterinary General Immunology and Microbiology Transmission (medicine) Water Pollution Oocysts Water Outbreak Toxoplasma gondii 04 agricultural and veterinary sciences General Medicine medicine.disease biology.organism_classification Toxoplasmosis Biological Assay Toxoplasma Brazil |
| Zdroj: | Transboundary and Emerging Diseases. 68:767-772 |
| ISSN: | 1865-1682 1865-1674 |
| Popis: | The protozoan Toxoplasma gondii is a causative agent of toxoplasmosis, an important and widespread zoonotic disease. The transmission of this disease in humans includes ingestion of sporulated oocysts present in contaminated water or food. T. gondii oocysts are widely distributed and toxoplasmosis is considered a major food- and waterborne pathogen worldwide, making drinking water containing sporulated T. gondii oocysts a major source of contamination for people. In the first half of 2018, an unprecedented outbreak of toxoplasmosis was reported in the city of Santa Maria, southern Brazil. The temporal and spatial distribution of the cases strongly suggested a waterborne infection. Thus, the aim of this study was to investigate a possible involvement of treated water as a source of the outbreak. For this, piglets received potentially contaminated water ad libitum for 21 days and the infection was monitored by serology through IFAT and investigation of T. gondii DNA in tissues by PCR amplification of a 529 bp followed by mouse bioassays. All piglets receiving test water ad libitum for 21 days as well as positive controls seroconverted to T. gondii. T. gondii DNA was detected in 62.5% of the piglets that received test water. All mice inoculated with tissues from each positive piglet were PCR-positive. These results strongly indicated the presence of viable oocysts in the test water administered to the animals during the study. |
| Databáze: | OpenAIRE |
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