Development of a multiparametric (bio)sensing platform for continuous monitoring of stress metabolites
| Autor: | Nicolas Verplanck, Pascal Mailley, Amir M. Ghaemmaghami, Simon Amigues, Karen Monsalve-Grijalba, Guillaume Costa, Ayman Chmayssem, Maxime Gougis, Véronique Mourier, Mélanie Alias, Constantin Edi Tanase, Séverine Vignoud |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Multiparametric Analysis
Chemistry business.industry Manufacturing process 010401 analytical chemistry Continuous monitoring Microfluidics Cell Culture Techniques 02 engineering and technology Biosensing Techniques Hydrogen Peroxide Modular design 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry Biochemical engineering Lactic Acid 0210 nano-technology business Nitrites |
| Zdroj: | Talanta |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2021.122275 |
| Popis: | There is a growing need for real-time monitoring of metabolic products that could reflect cell damages over extended periods. In this paper, we report the design and development of an original multiparametric (bio)sensing platform that is tailored for the real-time monitoring of cell metabolites derived from cell cultures. Most attractive features of our developed electrochemical (bio)sensing platform are its easy manufacturing process, that enables seamless scale-up, modular and versatile approach, and low cost. In addition, the developed platform allows a multiparametric analysis instead of single-analyte analysis. Here we provide an overview of the sensors-based analysis of four main factors that can indicate a possible cell deterioration problem during cell-culture: pH, hydrogen peroxide, nitric oxide/nitrite and lactate. Herein, we are proposing a sensors platform based on thick-film coupled to microfluidic technology that can be integrated into any microfluidic system using Luer-lock connectors. This platform allows obtaining an accurate analysis of the secreting stress metabolites during cell/tissues culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect