Reactive oxygen intermediates and eicosanoid production by kupffer cells and infiltrated macrophages in acute and chronic liver injury induced in rats by CCl4
| Autor: | L Alric, J.-C. Lepert, J.P. Vinel, M. Beraud, M. Duffaut, C Orfila, Nicolas Carrere, B. Pipy, G. Carrera |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Cirrhosis Kupffer Cells Immunology CCL4 Rats Sprague-Dawley chemistry.chemical_compound Liver disease Internal medicine medicine Macrophage Animals Pharmacology Liver injury Arachidonate 5-Lipoxygenase Arachidonic Acid biology Carbon Tetrachloride Poisoning Macrophages Zymosan medicine.disease Immunohistochemistry Rats Thromboxane B2 Endocrinology chemistry Arachidonate 5-lipoxygenase Acute Disease Chronic Disease biology.protein Eicosanoids Chemical and Drug Induced Liver Injury Reactive Oxygen Species Eicosanoid Production |
| Zdroj: | Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 49(12) |
| ISSN: | 1023-3830 |
| Popis: | Objective and Design: The aim of the present study was to characterize during acute and chronic liver injury induced by CCl4, macrophage phenotypes and whether a change in reactive oxygen intermediates (ROI) and eicosanoids production by Kupffer cells (KC) was observed.¶Material and Methods: Liver steato-necrosis and cirrhosis were induced in rats after 3 weeks and 9 weeks of CCl4 intoxication, respectively. Monocytes and tissue macrophages were identified by immunohistochemical study using monoclonal antibodies ED-1 and tissue macrophages using the antibody ED-2. The release of ROI and eicosanoids in response to the phorbol ester TPA (protein kinase activator) and to the calcium ionophore A23187 was assessed in cultivated cells.¶Results: As compared to healthy controls, livers of rats with steato-necrosis or cirrhosis exhibited a significant increase of ED-1 and ED-2 positive cells. Only KC from rats with liver steato-necrosis were found to have higher A23187, TPA + A23187 or opsonized zymosan induced ROI production than healthy controls (p |
| Databáze: | OpenAIRE |
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