ADP-induced changes in [32P]phosphate labeling of phosphatidylinositol-4,5-bisphosphate in washed rabbit platelets made refractory by prior ADP stimulation
| Autor: | J D Vickers, Raelene L. Kinlough-Rathbone, N. L. Leung, J F Mustard, H. J. Reimers |
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| Rok vydání: | 1983 |
| Předmět: |
Blood Platelets
Phosphatidylinositol 4 5-Diphosphate Phosphatidylinositol 4-phosphate Platelet Aggregation medicine.medical_treatment Biophysics Stimulation In Vitro Techniques Phosphatidylinositols Biochemistry Phosphates chemistry.chemical_compound Refractory medicine Animals Platelet Molecular Biology Chemistry Cell Biology Phosphate Adenosine Diphosphate Adenosine diphosphate Phosphatidylinositol 4 5-bisphosphate Isotope Labeling lipids (amino acids peptides and proteins) Rabbits Prostaglandin E |
| Zdroj: | Biochemical and biophysical research communications. 113(2) |
| ISSN: | 0006-291X |
| Popis: | Changes in 32P labeling of phosphatidylinositol-4,5-bisphosphate (PIP2) were examined during ADP-induced aggregation of washed rabbit platelets prelabeled with [32P]phosphate. ADP caused a significant decrease in the amount and 32P labeling of PIP2 at 10 and 60 sec. The decrease in labeling persisted at 2.5 min when the platelets were still aggregated, but disappeared by 10 min. Platelets refractory to ADP showed no further significant change in 32P in PIP2 when exposed to ADP; a decrease in PIP2 labeling could be induced, however, after platelets had recovered their disc shape and sensitivity to ADP. These data indicate that PIP2 may play a role in the response of platelets to ADP. |
| Databáze: | OpenAIRE |
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