High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector
| Autor: | M Dawood, C Lutze-Wallace, J Rommens, W Y Lui, P Wishart, Ernest Jay, D MacKnight, D Harrison, L Pomeroy-Cloney, V Asundi |
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| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Operon
Genetic Vectors Biology medicine.disease_cause law.invention Interferon-gamma law medicine Escherichia coli Genes Synthetic Humans Interferon gamma Amino Acid Sequence Cloning Molecular Gene Bacteriophage T5 Peptide sequence Cloning Multidisciplinary Base Sequence DNA Restriction Enzymes biology.organism_classification Molecular biology Genes Recombinant DNA medicine.drug Research Article |
| Popis: | A chemically synthesized gene for human interferon-gamma has been cloned into a prokaryotic expression vector under the regulation of a synthetic constitutive transcriptional-translational control unit that contains a strong bacteriophage T5 early promoter and a strong ribosome-binding site. Cells harboring the recombinant plasmid express high levels (4 X 10(9) units per liter of culture) of antiviral activity specific for interferon-gamma. Analysis of total cell lysates on NaDodSO4/polyacrylamide gels revealed a 17,200-dalton protein, expected for the nonglycosylated form of human interferon-gamma, that constitutes greater than 15% of total cell protein. |
| Databáze: | OpenAIRE |
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