Modified use of methylene blue in the tissue compression technique to detect sarcocysts in meat-producing animals
| Autor: | Yee Ling Lau, Yit Han Ng, Vellayan Subramaniam |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Diagnostic methods Meat Sarcocystosis Test sensitivity Food Contamination Biology Sensitivity and Specificity chemistry.chemical_compound medicine Animals General Veterinary Staining and Labeling Tissue compression Sarcocystis General Medicine Gold standard (test) biology.organism_classification Food Inspection Methylene Blue chemistry Parasitology Public Health Nested polymerase chain reaction Methylene blue |
| Zdroj: | Veterinary parasitology. 214(1-2) |
| ISSN: | 1873-2550 |
| Popis: | Sarcocystosis in meat-producing animals is a major cause of reduced productivity in many countries, especially those that rely on agriculture. Although several diagnostic methods are available to detect sarcocystosis, many are too time-consuming for routine use in abattoirs and meat inspection centers, where large numbers of samples need to be tested. This study aimed to compare the sensitivity of the methylene blue tissue preparation, unstained tissue preparation and nested PCR in the detection of sarcocysts in tissue samples. Approximately three-fold more sarcocysts were detected in methylene blue-stained tissue compared to unstained controls (McNemar's test: P |
| Databáze: | OpenAIRE |
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