The beneficial effect of EDTA on development of mouse one-cell embryos in chemically defined medium
| Autor: | Davor Solter, J. Abramczuk, Hilary Koprowski |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
C57BL/6
animal structures Zygote Cell Mice Inbred Strains Biology Andrology Mice medicine Animals Chelation Blastocyst Egtazic Acid Molecular Biology Edetic Acid Genetics Embryo Cell Biology biology.organism_classification Culture Media Chemically defined medium medicine.anatomical_structure embryonic structures Ectogenesis Female Developmental Biology |
| Zdroj: | Developmental Biology. 61:378-383 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/0012-1606(77)90308-6 |
| Popis: | An improved methology for culturing noninbred (ICR) mouse one-cell embryos is described. The successful development of one-cell embryos into blastocysts in chemically defined (Whitten's) medium was significantly enhanced by the presence of EDTA. More than 70% of ICR one-cell embryos developed into blastocysts in Whitten's medium in the presence of 10.8 μM EDTA, while, without EDTA, only 15–30% of embryos reached blastocyst stage. A concentration of 10.8 μM EDTA also promoted the development of 65–90% of inbred C57BL 6 one-cell embryos in Whitten's medium. This beneficial role of EDTA is probably related to the chelation of some metal ion(s) other than Ca2+ or Mg2+. |
| Databáze: | OpenAIRE |
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