Immunofluorescence localization of the Na-Ca exchanger in heart cells
| Autor: | G. E. Lindenmayer, W. J. Lederer, R. S. Kieval, Robert J. Bloch, A. Ambesi |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Physiology
Confocal Immunoblotting Immunocytochemistry Fluorescent Antibody Technique Immunofluorescence Sarcomere Sodium-Calcium Exchanger law.invention Confocal microscopy law medicine Fluorescence microscope Animals Tissue Distribution Sarcolemma medicine.diagnostic_test Chemistry Myocardium Cell Biology Anatomy Membrane Microscopy Fluorescence Biophysics Carrier Proteins Subcellular Fractions |
| Zdroj: | American Journal of Physiology-Cell Physiology. 263:C545-C550 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1992.263.2.c545 |
| Popis: | We investigated the localization of the Na-Ca exchanger in fixed, isolated heart cells from rat and guinea pig using immunocytochemical methods with epifluorescence and confocal microscopy. We found that the Na-Ca exchanger is distributed throughout all membranes in contact with the extracellular space, including the sarcolemma, the transverse tubules (T-tubules), and the intercalated disks. Microscopic nonuniformities in the fluorescent labeling appear to reflect varying views of the membranes containing Na-Ca exchanger protein. Confocal thin-section imaging reveals a regular grid of discrete foci of fluorescence, which represent Na-Ca exchanger in T-tubules viewed en face. These foci are 1.80 +/- 0.01 microns apart from sarcomere to sarcomere and are aligned with the Z-line. Along each Z-line, these foci are spaced at 1.22 +/- 0.11-microns intervals. Longitudinal sections of the sarcolemma-T-tubule junction show a comblike appearance, with T-tubules extending inward from the heavily labeled sarcolemma. Our finding that the Na-Ca exchanger is widely distributed over the cell surface may provide further insight into the role of Na-Ca exchange in the heart. |
| Databáze: | OpenAIRE |
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