Isolation of Decidual Lymphocytes From Chorionic Villus Samples: Phenotypic Analysis and Growth in Vitro
| Autor: | Mark K. Haynes, Maureen T. Flanagan, J. Bruce Smith, Bice Perussia, Laird G. Jackson |
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| Rok vydání: | 1995 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Cellular immunity T-Lymphocytes Lymphocyte T cell Immunology Population chemical and pharmacologic phenomena Cell Separation Biology Lymphocyte Activation Immunofluorescence Immunophenotyping Natural killer cell Antigens CD Pregnancy Decidua medicine Humans Immunology and Allergy education Cells Cultured education.field_of_study medicine.diagnostic_test Obstetrics and Gynecology hemic and immune systems Flow Cytometry Molecular biology CD56 Antigen Lymphocyte Subsets In vitro Killer Cells Natural Pregnancy Trimester First medicine.anatomical_structure Reproductive Medicine Interleukin-2 Female Chorionic Villi CD8 |
| Zdroj: | American Journal of Reproductive Immunology. 33:190-199 |
| ISSN: | 1046-7408 |
| DOI: | 10.1111/j.1600-0897.1995.tb00884.x |
| Popis: | PROBLEM: Giemsa stained cell isolates prepared from chorionic villus samples (CVS) contain granulated cells morphologically similar to large granular lymphocytes. METHOD: Phenotypic characterization of these cellular isolates by two-color immunofluorescence and subsequent in vitro culture in the presence of recombinant interleukin-2 (rIL-2) were done in order to determine whether CVS could serve as a source of decidual lymphocytes. RESULTS: A major fraction of the CVS-derived lymphocytes were characterized as decidual NK cells, exhibiting high levels of CD56 expression (CD56+bright), without concomitant expression of CD16. The T cell population present in CVS-derived lymphocytes contained both CD4+ and CD8+ cells in a ratio somewhat reduced compared to that found in peripheral blood. While both T cells and CD56+bright cells from CVS proliferate in vitro in response to rIL-2 alone, preferential growth of CD56+bnghtcells was accomplished using a selective culture technique wherein co-culture with an irradiated, B lymphoblastoid cell line promoted the growth of CD56+ cells. CONCLUSION: CVS contains decidual NK cells and T cells that proliferate in response to rIL-2 and/or third party stimulator cells. These culture techniques will allow investigations into the maturation and/or activation of decidual NK cells and T cells. |
| Databáze: | OpenAIRE |
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