Transcriptional repressors are increased in pancreatic islets of type 2 diabetic rats
| Autor: | Kinsuke Tsuda, Yu Ihara, Shinji Kagimoto, Yoshimichi Someya, Akira Kubota, Yutaka Seino, Yuichiro Yamada, Akari Inada, Akira Kuroe, Koichiro Yasuda |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Gene isoform
Male endocrine system medicine.medical_specialty CAMP-Responsive Element Modulator Transcription Genetic medicine.medical_treatment Biophysics Repressor Biology Biochemistry Rats Mutant Strains Cyclic AMP Response Element Modulator Islets of Langerhans Transcription (biology) Internal medicine Testis medicine Animals Insulin Protein Isoforms RNA Messenger Rats Wistar education Molecular Biology Regulation of gene expression education.field_of_study urogenital system Pancreatic islets Alternative splicing Cell Biology Rats DNA-Binding Proteins Repressor Proteins Endocrinology medicine.anatomical_structure Diabetes Mellitus Type 2 Gene Expression Regulation |
| Zdroj: | Biochemical and biophysical research communications. 253(3) |
| ISSN: | 0006-291X |
| Popis: | To further clarify the mechanism of impaired insulin gene transcription in the diabetic state, we investigated the expression and function of the transcriptional repressor CREM (CRE modulator) in rat pancreatic islets. The CREM gene generates both transcriptional activators and repressors by alternative splicing and an intronic promoter. We isolated a novel alternatively spliced CREM isoform, CREM-17X, which efficiently represses insulin gene transcription, in addition to the three previously reported repressors. We also compared mRNA levels of insulin and the CREM repressors in pancreatic islets of Wistar and GK (Goto-Kakizaki) rats, the well-characterized spontaneous animal model of type 2 diabetes. The CREM repressor levels are increased, and the expression of insulin mRNA is decreased in GK rats, suggesting that increased CREM repressor expression in pancreatic islets could contribute to the decreased insulin gene transcription that results in impaired insulin secretion in type 2 diabetes. |
| Databáze: | OpenAIRE |
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