Human cytomegalovirus-specific cytotoxic T cells. Relative frequency of stage-specific CTL recognizing the 72-kD immediate early protein and glycoprotein B expressed by recombinant vaccinia viruses
| Autor: | M P Cranage, J K Hickling, J G Sissons, Geoffrey L. Smith, L K Borysiewicz, S Graham, J H Sinclair |
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| Přispěvatelé: | Sinclair, John [0000-0002-2616-9571], Smith, Geoffrey [0000-0002-3730-9955], Apollo - University of Cambridge Repository |
| Rok vydání: | 1988 |
| Předmět: |
Human cytomegalovirus
Cytotoxicity Immunologic viruses Immunology Cytomegalovirus Fluorescent Antibody Technique chemical and pharmacologic phenomena Biology medicine.disease_cause Immediate early protein Herpesviridae Virus Immediate-Early Proteins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antigen Viral Envelope Proteins medicine Immunology and Allergy Cytotoxic T cell Humans Cloning Molecular Antigens Viral 030304 developmental biology 0303 health sciences Immunity Cellular virus diseases hemic and immune systems Articles medicine.disease Virology 3. Good health Clone Cells CTL chemistry Cytomegalovirus Infections Vaccinia Immunologic Memory 030215 immunology T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 0022-1007 |
| Popis: | CTL are held to be an important host defense mechanism in persistent herpes-virus infections. We have therefore studied the nature and specificity of human cytomegalovirus (HCMV)-specific CTL in normal persistently infected individuals. This was achieved by using vaccinia recombinants encoding viral genes expressed at different stages of the virus replicative cycle, a structural glycoprotein gB (vac.gB) and the major 72-kD immediate early nonstructural protein (vac.IE) of HCMV, combined with limiting dilution analysis of the CTL response. In two subjects, 43 and 58% of HCMV CTL precursors (CTLp) lysed vac.IE-infected cells, in contrast to less than 6% lysing gB-infected cells. HCMV-specific CTL could also be generated by secondary in vitro stimulation with vac.gB- but not vac.IE-infected autologous fibroblasts. The high frequency of 72-kD IE protein-specific CTL suggests that this is at least a major recognition element for the HCMV-specific CTL response in asymptomatic persistently infected individuals, and CTL with this specificity may be important in maintaining the normal virus/host equilibrium. |
| Databáze: | OpenAIRE |
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