Lymphocyte cytotoxicity and erythrocytic abnormalities induced in broiler chicks by fumonisins B1 and B2 and moniliformin from Fusarium proliferatum
| Autor: | Tariq Javed, Glenn A. Bennett, L. M. Cote, William B. Buck, J. L. Richard, Mary Ann Dombrink-Kurtzman |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Erythrocytes Cell Survival Veterinary (miscellaneous) Lymphocyte Fusarium proliferatum Applied Microbiology and Biotechnology Microbiology Fumonisins chemistry.chemical_compound Fusarium Fumonisin medicine Animals Viability assay Lymphocytes Fumonisin B2 Fumonisin B1 biology Broiler food and beverages Mycotoxins biology.organism_classification Animal Feed Carcinogens Environmental medicine.anatomical_structure chemistry Agronomy and Crop Science Moniliformin Chickens Cyclobutanes |
| Zdroj: | Mycopathologia. 124(1) |
| ISSN: | 0301-486X |
| Popis: | Peripheral blood lymphocytes were isolated from broiler chicks that had ingested feed amended with autoclaved Fusarium proliferatum culture material containing fumonisin B1 (FB1), fumonisin B2 (FB2) and moniliformin. Lymphocyte viability was determined for birds that were placed on amended rations at day 1 or day 7 of age at three different levels of mycotoxins, ranging from 61-546 ppm FB1, 14-94 ppm FB2 and 66-367 ppm moniliformin. Reduction of the tetrazolium salt, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], to yield MTT formazan, based on mitochondrial metabolic activity, was used to assess cell viability. Lymphocyte cytotoxic effects were observed in all treatment groups on day 21; chicks that started on amended feed at day 1 of age were affected more than those that started at day 7. Abnormal erythrocytes resembling early stages of erythroblasts were observed in peripheral blood from test chicks. Abnormally shaped red cells (poikilocytes) having a spindle-shape with one or both ends pointed were present. Some red cells appeared to be undergoing mitosis. Both reduced lymphocyte viability and abnormal erythrogenesis occurred in chicks given feed amended with F. proliferatum culture material containing FB1, FB2 and moniliformin. |
| Databáze: | OpenAIRE |
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