Highly sensitive and rapid detection of porcine circovirus 2 by avidin-biotin complex based lateral flow assay coupled to isothermal amplification
| Autor: | SeJin Kim, Sanghyo Kim, Junkyu Song, Minju Jang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Detection limit
Circovirus Avidin biotin complex biology Chemistry Swine General Chemical Engineering General Engineering Loop-mediated isothermal amplification Biotin biology.organism_classification Avidin Rapid detection Sensitivity and Specificity Analytical Chemistry Highly sensitive Porcine circovirus Molecular Diagnostic Techniques Biophysics Animals Naked eye Nucleic Acid Amplification Techniques |
| Zdroj: | Analytical methods : advancing methods and applications. 13(38) |
| ISSN: | 1759-9679 |
| Popis: | In this study, a new platform for the detection of porcine circovirus 2 was developed by avidin–biotin complex based lateral flow assay (LAMP-LFA). Improved detection sensitivity was attained by using loop mediated isothermal amplification (LAMP) with a low limit of detection (LOD), so the platform can be used to detect even early or asymptomatic stages of infection. LFA, which requires no specialized equipment, facilitates the use of point-of-care (POC) tests. Therefore, by applying LFA, the result can be confirmed accurately with the naked eye. Moreover, this platform has a unique structure using a single-tag detection system. The avidin–biotin interaction is the strongest interaction between proteins and has a higher Kd value than antigen–antibody interactions. Thus, the results are stable and can be clearly confirmed. The high sensitivity of LAMP-LFA enables all steps to be completed in 30 min. As a result, it could be applied to different targets, such as other pathogens. Future POC diagnostic studies are expected to be of great practical benefit. |
| Databáze: | OpenAIRE |
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