Persistence of immunoglobulin-producing cells in parotid salivary glands of patients with primary Sjogren's syndrome after B cell depletion therapy
| Autor: | Rodney P. E. Pollard, Frans G. M. Kroese, Erlin A. Haacke, Saravanan Yuvaraj, Fred K. L. Spijkervet, Annie Visser, Cees G. M. Kallenberg, Nicolaas A. Bos, Arjan Vissink, Nishath Hamza, Hendrika Bootsma |
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| Přispěvatelé: | Guided Treatment in Optimal Selected Cancer Patients (GUTS), Personalized Healthcare Technology (PHT), Translational Immunology Groningen (TRIGR) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_treatment Antibodies Monoclonal Murine-Derived DOUBLE-BLIND Immunology and Allergy Parotid Gland SYSTEMIC-LUPUS-ERYTHEMATOSUS B-Lymphocytes biology medicine.diagnostic_test Middle Aged Isotype medicine.anatomical_structure Lymphatic system Sjogren's Syndrome PLASMA-CELLS Rituximab Female Antibody medicine.drug Adult medicine.medical_specialty Adolescent BONE-MARROW Immunology General Biochemistry Genetics and Molecular Biology Lymphocyte Depletion Young Adult Rheumatology stomatognathic system Biopsy medicine Humans Immunologic Factors IMMUNOTHERAPY Antibody-Producing Cells IGA Aged business.industry MEMORY RITUXIMAB TREATMENT Immunotherapy Clone Cells Immunoglobulin A stomatognathic diseases LYMPHOID-TISSUE Immunoglobulin class switching Immunoglobulin G biology.protein Bone marrow business FOLLOW-UP |
| Zdroj: | Annals of the Rheumatic Diseases, 71(11), 1881-1887. BMJ PUBLISHING GROUP |
| ISSN: | 0003-4967 |
| Popis: | ObjectivesTo assess the persistence of immunoglobulin-producing cell populations in the parotid salivary glands of patients with primary Sjögren's syndrome (pSS) after B cell depletion therapy with rituximab.MethodsThirteen patients with pSS and four control patients were included in this study. Patients with pSS were treated with rituximab or placebo. Sequence analysis was carried out on IgA- and IgG-encoding transcripts extracted from parotid salivary gland biopsy specimens taken before treatment and at 12–16 and 36–52 weeks after treatment.ResultsAt baseline, many clonally related sequences were seen in patients with pSS. The number of clonal expansions was significantly higher in patients with pSS than in control patients. Clonal expansions were composed of IgA- and/or IgG-expressing cells. Rituximab did not significantly alter the degree of clonal expansions. Groups of clonally related cells had members which were shared between biopsy specimens taken before and after treatment. Mutation frequencies of immunoglobulin sequences from clonally related cells in patients with pSS were higher after treatment.ConclusionsRituximab treatment does not alter the characteristic features of increased clonal expansions seen in the parotid salivary glands of patients with pSS. The presence of clonally related immunoglobulin-producing cells before and after rituximab treatment strongly suggests that immunoglobulin-producing cells persist in salivary glands of patients with pSS despite B cell depletion. The presence of mixed isotype expression within groups of clonally related cells indicates local class switching in salivary glands of patients with pSS. Persistent immunoglobulin-producing cells may underlie disease relapse after treatment. |
| Databáze: | OpenAIRE |
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