Effects of IL-1β, IL-20, and BMP-2 on Intervertebral Disc Inflammation under Hypoxia
| Autor: | Ruey-Mo Lin, Kuo-Yuan Huang, Wen-Lung Liu, Yi-Shu Chiu, Yu Hsiang Hsu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
musculoskeletal diseases
Angiogenesis lcsh:Medicine Inflammation Article Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Interleukin 20 medicine 030304 developmental biology 0303 health sciences business.industry lcsh:R Interleukin Intervertebral disc General Medicine Hypoxia (medical) musculoskeletal system Vascular endothelial growth factor medicine.anatomical_structure chemistry inflammation 030220 oncology & carcinogenesis intervertebral disc (ivd) Cancer research medicine.symptom business inflammatory cytokines-20 (il-20) |
| Zdroj: | Journal of Clinical Medicine, Vol 9, Iss 1, p 140 (2020) Journal of Clinical Medicine Volume 9 Issue 1 |
| ISSN: | 2077-0383 |
| Popis: | Intervertebral disc (IVD) is an avascular tissue under hypoxic condition after adulthood. Our previous data showed that inflammatory cytokines (interleukin (IL)-1&beta ), IL-20, and bone morphogenetic protein-2 (BMP-2) play important roles in the healing process after disc injury. In the current study, we investigated whether IL-1&beta IL-20, or BMP-2 modulate the expression of pro-inflammatory cytokines, chemotaxis factor, and angiogenesis factor on IVD cells under hypoxia. IVD cells were isolated from patients with intervertebral disc herniation (HIVD) at the levels of L4&ndash 5 and L5&ndash S1. We found that the expression of IL-1&beta IL-20, BMP-2, hypoxia-inducible factor (HIF)-1&alpha IL-6, IL-8, angiogenetic factor (vascular endothelial growth factor (VEGF)), chemotactic factor (monocyte chemoattractant protein 1 (MCP-1)), and matrix metalloproteinase-3 (MMP-3) was upregulated in IVD cells under hypoxia conditions. In addition, IL-1&beta upregulated the expression of pro-inflammatory cytokines (IL-6 and IL-8), VEGF, MCP-1, and disc degradation factor (MMP-3) in IVD cells under hypoxia conditions. IL-20 upregulated MCP-1 and VEGF expression. BMP-2 also upregulated the expression of MCP-1, VEGF, and IL-8 in IVD cells under hypoxia conditions. Treatment with antibody against IL-1&beta decreased VEGF and MMP-3 expression, while treatment with IL-20 or BMP-2 antibodies decreased MCP-1, VEGF, and MMP-3 expression. Moreover, IL-1&beta modulated both the expression of IL-20 and BMP-2, but IL-20 only modulated BMP-2 either under a hypoxic or normoxic condition. Therefore, we concluded that the inflammation, chemotaxis, matrix degradation, and angiogenesis after disc herniation are influenced by the hypoxic condition and controlled by IL-1&beta IL-20, and BMP-2. |
| Databáze: | OpenAIRE |
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