Enkephalin Receptors and Receptor-Mediated Signal Transduction in Cultured Human Lymphocytes
| Autor: | W Heagy, Robert W. Finberg, Peter Lopez, Edna Teng |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
endocrine system
Enkephalin Enkephalin Methionine Lymphocyte Immunology Biology Pentapeptide repeat Jurkat cells Cell Line Immune system Cyclic AMP medicine Humans Lymphocytes Receptor Opioid peptide Naloxone Enkephalins Enkephalin Ala(2)-MePhe(4)-Gly(5) Molecular biology Immunopharmacology medicine.anatomical_structure Receptors Opioid Calcium Enkephalin D-Penicillamine (2 5) Signal Transduction |
| Zdroj: | Cellular Immunology. 191:34-48 |
| ISSN: | 0008-8749 |
| Popis: | Enkephalins are opioid peptides that bridge the neuroendocrine and immune systems. Using flow cytometry and a fluorescein conjugate of the endogenous pentapeptide methionine-enkephalin (ME), we have identified enkephalin receptors on cultured human lymphocytes. Cell surface recognition sites that bound ME with high affinity and specificity were detected for NALM 6 (pre-B acute lymphoblastic leukemia) and Jurkat (T lymphoma) cells. Brain-like enkephalin receptors were measured for these lymphocytes using conventional radioligand–receptor assays and the highly δ opioid receptor-selective enkephalin analog [3H]DPDPE. Upon activation, the lymphocyte enkephalin receptors transmitted signals that enhanced the accumulation of intracellular cAMP. These studies provide evidence that cultured human lymphocytes of the B (NALM 6 cells) and T (Jurkat cells) lineages express functional enkephalin receptors and suggest that such receptors may be instrumental in the lymphocyte response to opioid peptides and alkaloids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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