How Colloidal Nanoparticles Could Facilitate Multiplexed Measurements of Different Analytes with Analyte-Sensitive Organic Fluorophores

Autor:	Sebastian Friede, Jose-Maria Montenegro, Markus Ochs, Azhar Z. Abbasi, Wolfgang J. Parak, Tobias Niebling, Susana Carregal-Romero, Pilar Rivera Gil, Faheem Amin, Wolfram Heimbrodt
Rok vydání:	2011
Předmět:	Colloidal nanoparticles Analyte Förster resonance energy transfer Chemistry General Engineering General Physics and Astronomy Nanoparticle General Materials Science Nanotechnology Hybrid material Multiplexing Fluorescence
Zdroj:	ACS Nano. 5:21-25
ISSN:	1936-086X 1936-0851
DOI:	10.1021/nn1034026
Popis:	Multiplexed measurements of several analytes in parallel using analyte-sensitive organic fluorophores can be hampered by spectral overlap of the different fluorophores. The authors discuss how nanoparticles can help to overcome this problem. First, different organic fluorophores can be separated spatially by confining them to separate containers, each bearing a nanoparticle-based barcode. Second, by coupling different fluorophores to nanoparticles with different fluorescence lifetimes that serve as donors for excitation transfer, the effective fluorescence lifetime of the organic fluorophores as acceptors can be tuned by fluorescence resonance energy transfer (FRET). Thus, the fluorophores can be distinguished by their effective lifetimes. This is an example of how the modification of classical functional materials has already yielded improved and even new functionalities by the integration of nanoparticles with hybrid materials. We outline future opportunities in this area.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d106290027b49579e7d52a923b23828e https://doi.org/10.1021/nn1034026 Zobrazit plný text záznamu