Galactosyltransferase activity is not localized to the brush border membrane of human small intestine
| Autor: | Neil D. Cook, Tim J Peters, F. A. Boyle, Susan Snape, Paul Duane |
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| Rok vydání: | 1986 |
| Předmět: |
Brush border
Enterocyte Biophysics Biology Biochemistry Jejunum symbols.namesake Centrifugation Density Gradient medicine Humans Molecular Biology Galactosyltransferase Microvilli Cell Biology Golgi apparatus Galactosyltransferases Small intestine medicine.anatomical_structure Membrane symbols Cell fractionation Glucosidases Subcellular Fractions |
| Zdroj: | Bioscience Reports. 6:171-175 |
| ISSN: | 1573-4935 0144-8463 |
| DOI: | 10.1007/bf01115003 |
| Popis: | A recent report [Roth et al. (1985) J. Cell Biol.100: 118–125], using immunocytochemical techniques, calimed that human duodenal galactosyltransferase is located predominantly on the external aspect of enterocyte brush border membranes. Analytical subcellular fractionation by sucrose density gradient centrifugation of human jejunum biopsy homogenates demonstrated that galactosyltransferase activity is localized to the Golgi fraction (equilibrium density of 1.14 g cm−3) and is not found in significant amounts in the brush border membrane (equilibrium density of 1.22 g cm−3). |
| Databáze: | OpenAIRE |
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