Fully printed one-step biosensing device using graphene/AuNPs composite
| Autor: | Pedro Gómez-Romero, Alfredo de la Escosura-Muñiz, Marc Balsells, Bhawna Nagar, Arben Merkoçi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Working electrode
Materials science Biomedical Engineering Biophysics DNA Single-Stranded Metal Nanoparticles Nanotechnology Biosensing Techniques 02 engineering and technology Substrate (printing) 01 natural sciences Reference electrode law.invention Wax stamping Limit of Detection law Electrochemistry Screen printing Graphene Biosensing 010401 analytical chemistry Nucleic Acid Hybridization Equipment Design General Medicine DNA 021001 nanoscience & nanotechnology 0104 chemical sciences Dielectric spectroscopy Colloidal gold Dielectric Spectroscopy RGO Graphite Gold DNA Probes 0210 nano-technology Oxidation-Reduction Biosensor Biotechnology |
| Zdroj: | Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Biosensors and Bioelectronics Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Recercat. Dipósit de la Recerca de Catalunya instname |
| Popis: | Driven by the growing need of simple, cost efficient and flexible sensing systems, we have designed here a fully printed Reduced Graphene Oxide (rGO) based impedimetric sensor for one step sensing of DNA. The DNA sensor was fabricated by stamping of layered rGO and rGO/gold nanoparticles/single stranded DNA (rGO/AuNPs/ssDNA) composites over PET substrates using wax-printing technique. rGO works as an excellent working electrode, while the AuNPs create a suitable environment for ssDNA immobilization. Counter and reference electrodes were previously screen-printed on the plastic substrate, making thus a compact and highly integrated sensing platform. The change in electron transfer resistance after hybridization with a target ssDNA specific of Coxsackie B3 virus was monitored using electrochemical impedance spectroscopy (EIS), finding a linear response in the range of concentrations 0.01–20 µM. The novel, simple and straightforward one-step printing process for fabrication of a biosensing device developed keeps in mind the growing need of large scale device manufacturing. The successful proof-of-concept for the detection of DNA hybridization can be extended to other affinity biosensors, taking advantage of the integration of the bioreceptor on the sensor surface. Such ready-to-use biosensor would lead to a one-step electrochemical detection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|