Development of a polymerase chain reaction assay for Mycoplasma salivarium by using the nucleotide sequence within aminopeptidase My gene

Autor:	Tsuguo Watanabe, Yoshiaki Sato, Ken-ichiro Shibata, Yasunori Totsuka, Haruhisa Oguchi, Masayuki Kaga, Tetsuya Yoshikawa
Rok vydání:	2006
Předmět:	Adult DNA Bacterial Male Mycoplasma salivarium Sequence analysis ved/biology.organism_classification_rank.species medicine.disease_cause Aminopeptidases Polymerase Chain Reaction Sensitivity and Specificity Microbiology Cell Line law.invention Mice chemistry.chemical_compound Mycoplasma Bacterial Proteins stomatognathic system law Genetics medicine Animals Humans Mycoplasma Infections Child Saliva Molecular Biology Gene Polymerase chain reaction DNA Primers Bacteriological Techniques biology ved/biology Nucleic acid sequence Sequence Analysis DNA biology.organism_classification Molecular biology chemistry Child Preschool Mollicutes Female DNA
Zdroj:	FEMS Microbiology Letters. 156:281-286
ISSN:	0378-1097
DOI:	10.1111/j.1574-6968.1997.tb12741.x
Popis:	A polymerase chain reaction assay for a 278-nucleotide DNA fragment within aminopeptidase My gene of Mycoplasma salivarium was developed. The assay amplified M. salivarium DNA, but did not amplify DNAs of other mollicutes, bacteria and mammalian cells. The detection limit of the assay was 10 fg of DNA, approximately equivalent to 10 organisms.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1031b3bf4150bb04d27b90a22c99a2a https://doi.org/10.1111/j.1574-6968.1997.tb12741.x Zobrazit plný text záznamu Plný text