A SNP of HD-ZIP I transcription factor leads to distortion of trichome morphology in cucumber (Cucumis sativus L.)
| Autor: | Yue Chen, Keyan Zhang, Run Cai, Huanle He, Duo Lv, Haifan Wen, Jian Pan, Gang Wang, Leyu Zhang, Junsong Pan, Hui Du |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nuclear gene
Sequence analysis Mutant Genes Recessive Map-based cloning Plant Science Biology Trichome Genes Plant Polymorphism Single Nucleotide Genetic analysis Exon Gene Plant Proteins Genetics Cucumber Research Botany Trichomes biology.organism_classification QK1-989 Cucumis sativus Transcriptome Cucumis Transcription Factors |
| Zdroj: | BMC Plant Biology, Vol 21, Iss 1, Pp 1-13 (2021) BMC Plant Biology |
| ISSN: | 1471-2229 |
| Popis: | BackgroundTrichomes are excellent model systems for the analysis of cell differentiation and play essential roles in plant protection. From cucumber inbred line ‘WD1’, we identified an EMS-induced trichome abnormally developing mutant,nps, which exhibited smaller, denser and no pyramid-shaped head trichomes.ResultsUsing F2and BC1populations constructed from a cross betweennpsand ‘9930’, the genetic analysis showed that thenpstrait is controlled by a single recessive nuclear gene. We identifiedCsNpsby map-based cloning with 576 individuals of the F2population generated from the cross ofnpsand inbred line ‘9930’. TheCsNpswas located at a 13.4-kb genomic region on chromosome 3, which region contains three predicted genes. Sequence analysis showed that only one single nucleotide mutation (C → T) between 9930 andnpswas found in the second exon ofCsa3G748220, a plant-specific class I HD-Zip gene. The result of allelism test also indicated thatnpsis a novel allelic mutant ofMict(Micro-trichome). Thus,npswas renamedmict-L130F. By comparing the transcriptome ofmict-L130Fvs WD1 and 06–2 (mict) vs 06–1 (wildtype, near-isogenic line of 06–2), several potential target genes that may be related to trichome development were identified.ConclusionsOur results demonstrate thatMict-L130Fis involved in the morphogenesis of trichomes. Map-based cloning of theMict-L130Fgene could promote the study of trichome development in cucumber. |
| Databáze: | OpenAIRE |
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