DIFFERENCE IN DEGREE OF SUB-LETHAL DAMAGE RECOVERY BETWEEN CLINICAL PROTON BEAMS AND X-RAYS
| Autor: | Takeji Sakae, Takamitsu A. Kato, Yoshitaka Matsumoto, Koji Tsuboi, Yuta Sekino, Koichi Ando, Hitoshi Ishikawa, Hideyuki Sakurai |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
DNA Repair
Proton Cell Survival Kinetics Linear energy transfer In Vitro Techniques Radiation 030218 nuclear medicine & medical imaging 03 medical and health sciences Cricetulus 0302 clinical medicine Proton Therapy Relative biological effectiveness Animals DNA Breaks Double-Stranded Linear Energy Transfer Radiology Nuclear Medicine and imaging Irradiation Proton therapy Cells Cultured Radiological and Ultrasound Technology Chemistry X-Rays Public Health Environmental and Occupational Health Dose-Response Relationship Radiation General Medicine Immunohistochemistry Dose–response relationship Microscopy Fluorescence 030220 oncology & carcinogenesis Biophysics Relative Biological Effectiveness |
| Zdroj: | Radiation Protection Dosimetry. 183:93-97 |
| ISSN: | 1742-3406 0144-8420 |
| DOI: | 10.1093/rpd/ncy270 |
| Popis: | Fractionated proton beam radiotherapy is spreading worldwide these days. However, biological data of sub-lethal damage recovery (SLDR) after proton irradiation is not known yet. We here conducted split-dose experiments (20-360 min intervals) to clarify SLDR kinetics, and also compared the kinetics between cells with different repairability of DNA double-strand breaks. CHO and 51D1 cell lines but not V3 cell line showed significant SLDR, which reached plateau in 4-6 h. The recovery rates and recovery halftime of SLDR after X-rays were significantly higher and shorter than proton beams for CHO and 51D1 cells, respectively. Additionally, the frequency of remaining gamma-H2AX foci after two fractions was remarkably higher for X-rays than proton beams. These data suggest that there is a difference between proton beam and X-rays in SLDR and the retained DNA double-strand breaks after split-dose irradiation. |
| Databáze: | OpenAIRE |
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