Evaluation of high-throughput assays for in vitro drug susceptibility testing of Tritrichomonas foetus trophozoites
| Autor: | Kylie Thompson, Steve A. Carlson, Chris Bader, Cindy Hall, Matthew T. Brewer, Jeba Jesudoss Chelladurai |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
High-throughput screening Tritrichomonas foetus Microbiology 03 medical and health sciences chemistry.chemical_compound High-Throughput Screening Assays medicine Trophozoites Propidium iodide Anthelmintics Trichomoniasis Dose-Response Relationship Drug General Veterinary biology Optical Imaging General Medicine 030108 mycology & parasitology biology.organism_classification medicine.disease In vitro Metronidazole chemistry Luminescent Measurements Immunology Parasitology Ronidazole medicine.drug |
| Zdroj: | Veterinary Parasitology. 223:34-37 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2016.04.006 |
| Popis: | Tritrichomonas foetus is a sexually transmitted protozoan parasite that causes abortions in cattle and results in severe economic losses. In the United States, there are no safe and effective treatments for this parasite and infected animals are typically culled. In order to expedite drug discovery efforts, we investigated in vitro trophozoite killing assays amenable to high-throughput screening in 96 well plate formats. We evaluated the reduction of resorufin, incorporation of propidium iodide, and a luminescence-based ATP detection assay. Of these methods, reduction of resorufin was found to be the most reliable predictor of trophozoite concentrations. We further validated this method by conducting dose-response experiments suitable for calculation of EC50 values for two established compounds with known activity against trophozoites in vitro, namely, metronidazole and ronidazole. Our results demonstrate that the resorufin method is suitable for high-throughput screening and could be used to enhance efforts targeting new treatments for bovine trichomoniasis. |
| Databáze: | OpenAIRE |
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