In Vitro Activities of a Novel Nanoemulsion against Burkholderia and Other Multidrug-Resistant Cystic Fibrosis-Associated Bacterial Species
| Autor: | John J. LiPuma, Paul E. Makidon, James R. Baker, Linda M. Kalikin, Bridget K. Foster, Jordan C. Keoleian, Sivaprakash Rathinavelu |
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| Rok vydání: | 2009 |
| Předmět: |
Pharmacology
Achromobacter Cystic Fibrosis biology Burkholderia Sputum Microbial Sensitivity Tests Acinetobacter biology.organism_classification Anti-Bacterial Agents Microbiology Multiple drug resistance Burkholderia cepacia complex Infectious Diseases Antibiotic resistance Susceptibility Biofilms Drug Resistance Multiple Bacterial Pandoraea Humans Nanoparticles Emulsions Pharmacology (medical) Stenotrophomonas |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 53:249-255 |
| ISSN: | 1098-6596 0066-4804 |
| Popis: | Respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (CF). Species within the Burkholderia cepacia complex are especially problematic in this patient population. We investigated a novel surfactant-stabilized oil-in-water nanoemulsion (NB-401) for activity against 150 bacterial isolates recovered primarily from CF respiratory tract specimens. These specimens included 75 Burkholderia isolates and 75 isolates belonging to other CF-relevant species including Pseudomonas , Achromobacter , Pandoraea , Ralstonia , Stenotrophomonas , and Acinetobacter . Nearly one-third of the isolates were multidrug resistant, and 20 (13%) were panresistant based on standard antibiotic testing. All isolates belonging to the same species were genotyped to ensure that each isolate was a distinct strain. The MIC 90 of NB-401 was 125 μg/ml. We found no decrease in activity against multidrug-resistant or panresistant strains. MBC testing showed no evidence of tolerance to NB-401. We investigated the activity of NB-401 against a subset of strains grown as a biofilm and against planktonic strains in the presence of CF sputum. Although the activity of NB-401 was decreased under both conditions, the nanoemulsion remained bactericidal for all strains tested. These results support NB-401's potential role as a novel antimicrobial agent for the treatment of infection due to CF-related opportunistic pathogens. |
| Databáze: | OpenAIRE |
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