Effects of Administration of Hydrocortisone on the Renal and Biliary Excretion of Ampicillin in the Dog
| Autor: | Sergio Mandiola, B. L. Johnson, James Dahlgren |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Taurocholic Acid
medicine.medical_specialty Hydrocortisone In Vitro Techniques Kidney Diffusion Excretion chemistry.chemical_compound Dogs Internal medicine Ampicillin Infusion Procedure medicine Animals Bile Immunology and Allergy Chromatography Creatinine Chemistry biochemical phenomena metabolism and nutrition Infectious Diseases Endocrinology medicine.anatomical_structure Liver Sephadex Hepatocyte Protein Binding medicine.drug |
| Zdroj: | Journal of Infectious Diseases. 129:37-44 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/129.1.37 |
| Popis: | Hydrocortisone produced choleresis in anesthetized dogs receiving taurocholate and ampicillin. There was an increase in mean concentration of ampicillin in serum after infusion of hydrocortisone with no change in mean rate of biliary excretion of ampicillin. In dogs receiving creatinine in addition to taurocholate and ampicillin, hydrocortisone also produced an increase in mean levels of ampicillin in serum. The mean rate of biliary excretion and the rate of renal clearance of ampicillin were constant in these dogs. In dogs receiving creatinine, taurocholate, and ampicillin without infusion of hydrocortisone, mean concentration of ampicillin in serum, minute biliary weight, and renal clearance rate of ampicillin remained constant throughout the 150 min of study, while mean biliary concentration of ampicillin and rate of excretion decreased during the last 40 min. The increased concentrations of ampicillin in serum of dogs during and after infusion of hydrocortisone seems due to decreased diffusion of ampicillin from plasma into the hepatocyte. Preliminary chromatographic studies of rat liver supernate on Sephadex G-75 indicate that bioactivity of ampicillin appears at a position in the elution pattern where Y protein should be eluted. Y protein binds anoinic metabolites of cortisol. Competitive or noncompetitive inhibition by hydrocortisone of binding of ampicillin to Y protein could explain the increased concentrations in serum during and after infusion of hydrocortisone. |
| Databáze: | OpenAIRE |
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