Regulation of an aspartyl-tRNA species in BHK cells in culture and in solid tumor form. II. The effect of different sera on aspartyl-tRNA in culture
| Autor: | D.L. Adams, M.V. Marshall, A.C. Griffin |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Ascitic fluid
Cancer Research Fetus Kidney Aspartic Acid Chromatography Hamster Neoplasms Experimental Biology RNA Transfer Amino Acyl Culture Media Mice medicine.anatomical_structure Oncology Biochemistry Liver Aspartic acid Transfer RNA Baby hamster kidney cell medicine Animals RNA Neoplasm Solid tumor Cells Cultured |
| Zdroj: | Cancer letters. 4(3) |
| ISSN: | 0304-3835 |
| Popis: | In normal tissues aspartyl tRNA is present in 3 separable components by RPC-5-chromatography, 2 small and early eluting peaks and a major component designated rRNAAspIII. A late eluting peak, tRNAAspIV has also been found in many tumors of human and animals origin, and in BHK 21/13 cells grown in culture, where 27% or more of the total aspartate acceptance occurs in this peak. In contrast, normal tissue studied, i.e., hamster liver or kidney, exhibits less than 2.0% of the total aspartyl tRNA as the late eluting tRNAAspIV. Increasing the level of fetal calf serum in the medium from 5 to 15% resulted in a change from 0.79 to 1.83 in the ratio of tRNAAspIII--tRNAAspIV in BHK cells. Utilizing sera from different species, the percentage of tRNAAspIV was found to vary between 26.8 and 66.0% while the combined percentages of tRNAAspIII and tRNAAspIV varied between 59.5 and 80.8%. It appears that the increased tRNAAspIII was derived from tRNAAspIV and vice versa indicating that there may be a close structural relationship between tRNAAspIII and tRNAAspIV. It was also observed that BHK tumor cells or ascitic fluid enhanced the appearance of tRNAAspIV in normal host liver. |
| Databáze: | OpenAIRE |
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