A role of salivary carbonic anhydrase VI in dental plaque
| Autor: | Yuzo Ogawa, Masaya Kimoto, Mitsunobu Kishino, Yoshiaki Yura |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Sucrose Saliva Bicarbonate Blotting Western Dental Plaque Dental plaque Immunoenzyme Techniques chemistry.chemical_compound Western blot Carbonic anhydrase medicine Humans Carbonic Anhydrase Inhibitors General Dentistry Carbonic Anhydrases medicine.diagnostic_test biology Cell Biology General Medicine Hydrogen-Ion Concentration medicine.disease Molecular biology Acetazolamide Blot Protein Subunits Otorhinolaryngology chemistry Biochemistry biology.protein Female medicine.drug |
| Zdroj: | Archives of Oral Biology. 51:117-122 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2005.04.007 |
| Popis: | Summary Objective Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO 2 + H 2 O ↔ H + + HCO 3 − . CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva. Design Nine healthy subjects participated in the study. The pH of the dental plaque from each subject was monitored after a mouth rinse with 10% sucrose with or without 10 −5 M acetazolamide, a specific inhibitor of CA. Also CA was examined in plaque by enzyme histochemistry, immunohistochemistry and Western blot analysis. Results Though sucrose and sucrose plus inhibitor yielded Stephan curves with a similar temporal pattern, the pH values of the latter were significantly lower than those of the former. Plaque exhibited CA activity by enzyme histochemistry. Immunohistochemistry and Western analysis demonstrated that the activity was due to CA VI but not to CA I or CA II. Conclusions The results indicate that CA VI in saliva penetrates plaque and facilitates acid neutralization by salivary bicarbonate. Therefore, CA VI may be considered an anti-caries protein in saliva. |
| Databáze: | OpenAIRE |
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