Protein Identification of Venoms of the African Spitting Cobras, Naja mossambica and Naja nigricincta nigricincta
| Autor: | Erastus H. Haindongo, Peter James, Ottilie Katali, Loide Ndelimona Ndapandula Shipingana, Timothy Rennie, Mirva Pääkkönen, Christian J. Hunter, John E. Eriksson, Peter Nyarango |
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| Rok vydání: | 2020 |
| Předmět: |
Proteome
Protein family Health Toxicology and Mutagenesis Naja Antivenom lcsh:Medicine Venom Reptilian Proteins Toxicology complex mixtures Article Naja mossambica 03 medical and health sciences Tandem Mass Spectrometry Naja nigricincta nigricincta Animals snakebites Envenomation Chromatography High Pressure Liquid Naja nigricincta snake venom 030304 developmental biology Elapid Venoms 0303 health sciences biology lcsh:R 030302 biochemistry & molecular biology African spitting Cobras antivenin biology.organism_classification Secretory protein Biochemistry Snake venom |
| Zdroj: | Toxins, Vol 12, Iss 520, p 520 (2020) Toxins Volume 12 Issue 8 |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins12080520 |
| Popis: | Cobra snakes, including Naja mossambica and Naja nigricincta nigricincta, are one of the major groups of snakes responsible for snakebites in southern Africa, producing significant cytotoxicity and tissue damage. The venom of N. mossambica has been briefly characterised, but that of N. n. nigricincta is not reported. The current study identifies the venom proteins of N. mossambica and N. n. nigricincta. This is achieved using sodium dodecyl sulphate (SDS)-polyacrylamide gel eletrophroresis (PAGE), followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Most of the proteins were less than 17 kDa in both snakes. N. mossambica was found to have 75 proteins in total (from 16 protein families), whereas N.n. nigricincta had 73 (from 16 protein families). Of these identified proteins, 57 were common in both snakes. The proteins identified belonged to various families, including the three-finger toxins (3FTx), Cysteine-rich secretory proteins (CRiSP), Phospholipase A2 (PLA2) and Venom metalloproteinase M12B (SVMP). The current study contributes to the profile knowledge of snake venom compositions, which is of fundamental value in understanding the proteins that play a major role in envenomation. |
| Databáze: | OpenAIRE |
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