Integrated analysis of physiological, transcriptomics and metabolomics provides insights into detoxication disruption of PFOA exposure in Mytilus edulis
| Autor: | Sun Xiaojie, Yongxing Yu, Mengmeng Guo, Yanhua Jiang, Zhaoxin Li, Zhijun Tan, Yuxiu Zhai, Meng Qu, Fengling Li, Yao Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Mytilus edulis
Health Toxicology and Mutagenesis 0211 other engineering and technologies 02 engineering and technology 010501 environmental sciences Carbohydrate metabolism 01 natural sciences Antioxidants Environmental pollution Transcriptome chemistry.chemical_compound Metabolomics Detoxification Animals GE1-350 Protein kinase A Omics analysis 0105 earth and related environmental sciences 021110 strategic defence & security studies Fluorocarbons Public Health Environmental and Occupational Health Lipid metabolism General Medicine Peroxisome Lipid Metabolism Pollution PPAR gamma Environmental sciences chemistry Biochemistry TD172-193.5 Oxidative stress Perfluorooctanoic acid Caprylates |
| Zdroj: | Ecotoxicology and Environmental Safety, Vol 214, Iss, Pp 112081-(2021) |
| ISSN: | 0147-6513 |
| Popis: | Perfluorooctanoic acid (PFOA), a persistent environmental contaminant, resists environmental degradation and bioaccumulates in food chains. Lots of literatures have proved that PFOA exposure could disrupt detoxifying function in a variety of organisms, however, it still remained poorly known about this in mollusk. Here, we examined physiological, transcriptomic, and metabolomic responses to PFOA in Mytilus edulis, a model organism frequently used in studies of aquatic pollution. We aimed to characterize PFOA-induced stress responses and detoxification mechanisms. PFOA exposure significantly altered antioxidant enzyme activity levels and the abundances of lipid peroxidation products. In addition, transcriptomic analysis indicated that several genes associated with oxidative stress and detoxication were differentially expressed after PFOA exposure. In combination, transcriptomic and metabolomic analyses showed that PFOA exposure disturbed several metabolic processes in M. edulis, including the lipid metabolism, amino acid metabolism, and carbohydrate metabolism etc. Molecular examination and enzymes assay of PFOA-exposed M. edulis after a 7-day depuration period still did not recover to control levels. The Pathway enrichment analysis proved that several pathways related to detoxification, such as c-Jun N-terminal kinase (JNK) and p38-dependent mitogen-activated protein kinase (MAPK) pathway, Peroxisome proliferator-activated receptor γ (PPARγ) pathway etc, were obviously affected. The present work verifies firstly PFOA disruption to molluscan detoxification and identifies the key pathways to understand the molecular mechanisms thereof. This study provides new insights into the detoxication disruption invoked in response to PFOA exposure in M. edulis. |
| Databáze: | OpenAIRE |
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