Magnetic nanoparticle hyperthermia enhances radiation therapy: A study in mouse models of human prostate cancer
| Autor: | Cila Herman, Christine Cornejo, Anilchandra Attaluri, Haoming Zhou, Theodore L. DeWeese, Mohammad Hedayati, Sri Kamal Kandala, Robert Ivkov, Michael Armour, Yonggang Zhang, Michele Wabler |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Hyperthermia
Male Cancer Research Pathology medicine.medical_specialty Radiation-Sensitizing Agents Physiology medicine.medical_treatment Magnetic Field Therapy Article Mass Spectrometry Prostate cancer chemistry.chemical_compound Mice Prostate Physiology (medical) Cell Line Tumor medicine Distribution (pharmacology) Animals Humans Magnetite Nanoparticles business.industry Chemistry Cancer Prostatic Neoplasms Hyperthermia Induced medicine.disease Combined Modality Therapy Radiation therapy medicine.anatomical_structure Magnetic nanoparticles Nuclear medicine business Iron oxide nanoparticles |
| Popis: | We aimed to characterise magnetic nanoparticle hyperthermia (mNPH) with radiation therapy (RT) for prostate cancer.Human prostate cancer subcutaneous tumours, PC3 and LAPC-4, were grown in nude male mice. When tumours measured 150 mm3 magnetic iron oxide nanoparticles (MIONPs) were injected into tumours to a target dose of 5.5 mg Fe/cm3 tumour, and treated 24 h later by exposure to alternating magnetic field (AMF). Mice were randomly assigned to one of four cohorts to characterise (1) intratumour MIONP distribution, (2) effects of variable thermal dose mNPH (fixed AMF peak amplitude 24 kA/m at 160 ± 5 kHz) with/without RT (5 Gy), (3) effects of RT (RT5: 5 Gy; RT8: 8 Gy), and (4) fixed thermal dose mNPH (43 °C for 20 min) with/without RT (5 Gy). MIONP concentration and distribution were assessed following sacrifice and tissue harvest using inductively coupled plasma mass spectrometry (ICP-MS) and Prussian blue staining, respectively. Tumour growth was monitored and compared among treated groups.LAPC-4 tumours retained higher MIONP concentration and more uniform distribution than did PC3 tumours. AMF power modulation provided similar thermal dose for mNPH and combination therapy groups (CEM43: LAPC-4: 33.6 ± 3.4 versus 25.9 ± 0.8, and PC3: 27.19 ± 0.7 versus 27.50 ± 0.6), thereby overcoming limitations of MIONP distribution and yielding statistically significant tumour growth delay.PC3 and LAPC-4 tumours represent two biological models that demonstrate different patterns of nanoparticle retention and distribution, offering a model to make comparisons of these effects for mNPH. Modulating power for mNPH offers potential to overcome limitations of MIONP distribution to enhance mNPH. |
| Databáze: | OpenAIRE |
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