Paraoxonase activity is reduced by a pro-atherosclerotic diet in rabbits
| Autor: | M, Mackness, A, Boullier, N, Hennuyer, B, Mackness, M, Hall, A, Tailleux, P, Duriez, B, Delfly, P, Durrington, J C, Fruchart, N, Duverger, J M, Caillaud, G, Castro, A, Bouiller |
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| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Arteriosclerosis Transgene Lipoproteins Phospholipid Biophysics Biochemistry Animals Genetically Modified chemistry.chemical_compound Internal medicine medicine Animals Humans Serum paraoxonase Molecular Biology Apolipoprotein A-I business.industry Cholesterol Human apolipoprotein Aryldialkylphosphatase Paraoxonase activity Cholesterol HDL Esterases Cell Biology PON1 Lipids Lipoproteins LDL Endocrinology chemistry Diet Atherogenic lipids (amino acids peptides and proteins) Rabbits business Oxidation-Reduction Lipoprotein |
| Zdroj: | Biochemical and biophysical research communications. 269(1) |
| ISSN: | 0006-291X |
| Popis: | Serum paraoxonase (PON1) is believed to protect against the development of atherosclerosis because of its ability to retard the oxidation of low-density lipoprotein (LDL) by hydrolysing LDL-associated phospholipid and cholesteryl-ester hydroperoxides. We have examined the relationship between PON1 and atherosclerosis development in transgenic rabbits overexpressing human apolipoprotein (apo) A-I and nontransgenic littermates fed a pro-atherogenic diet. PON1 activity was higher in transgenic (4006.1 +/- 716.7 nmol/min/ml) compared to control (3078.5 +/- 623.3 nmol/min/ml) rabbits (P0.01) while high-density lipoprotein (HDL) cholesterol was 1.84 +/- 0.54 mmol/L in transgenic rabbits and 0.57 +/- 0.21 mmol/L in control rabbits (P = 0.0001). After feeding rabbits a high-cholesterol diet for 14 weeks HDL-cholesterol fell by 70% in both transgenic and control rabbits (P0.001 compared to week 0) PON1 activity fell by 50% in both groups of rabbits (P0. 01 compared to week 0). The amount of thoracic aortic surface area covered by lesions was 29 +/- 16% in the control group and 26 +/- 15% in the transgenic group (P = NS). A pro-atherosclerotic diet reduces PON1 which may exaggerate the effects of the diet on the development of atherosclerosis. |
| Databáze: | OpenAIRE |
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