Karyotypic variation and pollen stainability in resynthesized allopolyploids Tragopogon miscellus and T. mirus
| Autor: | Evgeny V. Mavrodiev, Roseana Rodriguez, Pamela S. Soltis, Jonathan P. Spoelhof, Kweon Heo, Michael Chester, Douglas E. Soltis, Blake Geraci |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Karyotype Plant Science medicine.disease_cause Tragopogon Polyploidy 03 medical and health sciences Pollen Gene duplication Botany Genetics medicine Tragopogon miscellus Ecology Evolution Behavior and Systematics In Situ Hybridization biology Tragopogon mirus Genetic Variation biology.organism_classification Diploidy 030104 developmental biology Variation (linguistics) Genetics Population Evolutionary biology Karyotyping Rapid onset Ploidy Genome Plant |
| Zdroj: | American journal of botany. 104(10) |
| ISSN: | 1537-2197 |
| Popis: | PREMISE OF THE STUDY Polyploidy has extensively shaped the evolution of plants, but the early stages of polyploidy are still poorly understood. The neoallopolyploid species Tragopogon mirus and T. miscellus are both characterized by widespread karyotypic variation, including frequent aneuploidy and intergenomic translocations. Our study illuminates the origins and early impacts of this variation by addressing two questions: How quickly does karyotypic variation accumulate in Tragopogon allopolyploids following whole-genome duplication (WGD), and how does the fertility of resynthesized Tragopogon allopolyploids evolve shortly after WGD? METHODS We used genomic in situ hybridization and lactophenol-cotton blue staining to estimate the karyotypic variation and pollen stainability, respectively, of resynthesized T. mirus and T. miscellus during the first five generations after WGD. KEY RESULTS Widespread karyotypic variation developed quickly in synthetics and resembled that of naturally occurring T. mirus and T. miscellus by generation S4 . Pollen stainability in resynthesized allopolyploids was consistently lower than that of natural T. mirus and T. miscellus, as well as their respective diploid progenitor species. Logistic regression showed that mean pollen stainability increased slightly over four generations in resynthesized T. mirus but remained at equivalent levels in T. miscellus. CONCLUSIONS Our results clarify some of the changes that occur in T. mirus and T. miscellus immediately following their origin, most notably the rapid onset of karyotypic variation within these species immediately following WGD. |
| Databáze: | OpenAIRE |
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