Transplantation of rabbit kidneys perfused with glycerol solutions at 10 degrees C
| Autor: | I.A. Jacobsen, M.C. Wusteman, E. Pegg, S.M. Robinson |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Glycerol
Renal function General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound medicine Animals Mannitol Creatinine Chromatography Osmolar Concentration Temperature General Medicine Kidney Transplantation Transplantation Perfusion chemistry Biochemistry Polygeline Urea Rabbits General Agricultural and Biological Sciences Haemaccel medicine.drug |
| Zdroj: | Cryobiology. 15(1) |
| ISSN: | 0011-2240 |
| Popis: | Experiments are described in which rabbit kidneys were perfused with solutions containing 2, 3, or 4 m glycerol and then were autografted in order to measure any functional impairment. The basic perfusate contained Haemaccel (17.5 g/litre) and mannitol (111 m m ) and had a total osmolality of 400 mosmol/kg. The perfusion temperature was 10 °C. Glycerol concentration was slowly increased at a rate of 30 m m min−1 to 2, 3, or 4 m , was held at the maximum concentration for 30 min, and was then decreased to m at 30 m m min−1. An immediate contralateral nephrectomy was carried out, and the function of the perfused kidney was assessed by serial measurements of blood urea and serum creatinine, by the determination of 24-hr endogenous creatinine clearance at 1 month and by histological examination. It was found that a concentration of 3 m glycerol was readily tolerated. |
| Databáze: | OpenAIRE |
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