A Novel Combination of Wheat Peptides and Fucoidan Attenuates Ethanol-Induced Gastric Mucosal Damage through Anti-Oxidant, Anti-Inflammatory, and Pro-Survival Mechanisms

Autor: Molly M. Hood, Min Gui, Jeff Scholten, Xingchang Pan, Juntao Kan, Jun Du, Feng Tian, Charlie R. Burns, Jennifer Chuang
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Time Factors
Protein Hydrolysates
Anti-Inflammatory Agents
Pharmacology
Ulcer index
Antioxidants
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
fucoidan
Epidermal growth factor
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Triticum
Plant Proteins
Nutrition and Dietetics
medicine.diagnostic_test
Fucoidan
anti-oxidant
Stomach
ErbB Receptors
medicine.anatomical_structure
030211 gastroenterology & hepatology
Gastritis
medicine.symptom
pro-survival
lcsh:Nutrition. Foods and food supply
Signal Transduction
Cell Survival
lcsh:TX341-641
Caspase 3
Biology
Article
Cell Line
Superoxide dismutase
03 medical and health sciences
gastric mucosal damage
wheat peptides
anti-inflammation
Western blot
Polysaccharides
medicine
Animals
Humans
Stomach Ulcer
Dose-Response Relationship
Drug
Ethanol
Disease Models
Animal
030104 developmental biology
chemistry
Gastric Mucosa
Immunology
biology.protein
Biomarkers
Food Science
Zdroj: Nutrients; Volume 9; Issue 9; Pages: 978
Nutrients, Vol 9, Iss 9, p 978 (2017)
Nutrients
ISSN: 2072-6643
DOI: 10.3390/nu9090978
Popis: Gastritis or peptic ulcer is believed to affect about half of people worldwide. Traditional medications can lead to adverse effects, therefore, alternative nutritional strategies are needed to prevent the development of gastric mucosal damage. A novel combination of two food-grade ingredients, wheat peptides and fucoidan (WPF), was prepared to treat male Sprague Dawley rats for 30 days before gastric mucosal damage was induced by oral administration of ethanol. The serum levels of biomarkers were determined by enzyme-linked immunosorbent assay. Biomarkers in stomach tissue were analyzed using immunohistochemistry. In addition, human gastric epithelial cell line (GES-1) was used to investigate protein expression by Western blot. WPF could attenuate ethanol-induced gastric mucosal damage in an inverse dose-dependent manner, with both ulcer index and pathological index improved. WPF increased superoxide dismutase level and decreased malondialdehyde level. WPF also decreased the levels of interleukin-8, platelet-activating factor, and Caspase 3, while increasing the levels of prostaglandin E-2, epidermal growth factor (EGF), and EGF receptor (EGFR). Furthermore, phosphorylation of EGFR and extracellular signal–regulated kinases was induced by WPF in GES-1 cells. In conclusion, the novel combination of wheat peptides and fucoidan attenuated ethanol-induced gastric mucosal damage in rats through anti-oxidant, anti-inflammatory, and pro-survival mechanisms.
Databáze: OpenAIRE
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