Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children

Autor: Asger M. Frank, Lea Barfod, Frederica D. Partey, Melanie R. Walker, Michael F. Ofori, Lars Hviid, Filip C. Castberg, Anne Knudsen, Edem W. Sarbah, Maria Rosaria Bassi
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Plasmodium
Quantitative Parasitology
Physiology
Antibodies
Protozoan
Antibody Response
Parasitemia
Biochemistry
Medical Conditions
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
030212 general & internal medicine
Malaria
Falciparum
Child
Immune Response
Protozoans
Immune System Proteins
Multidisciplinary
biology
Malaria vaccine
Malarial Parasites
Eukaryota
Antibody Isotype Determination
Middle Aged
Child
Preschool
Medicine
Female
Antibody
Research Article
Adult
Adolescent
Science
Plasmodium falciparum
Immunology
Antigens
Protozoan
Research and Analysis Methods
Antibodies
Young Adult
03 medical and health sciences
Immune system
Antigen
Malaria Vaccines
Parasite Groups
parasitic diseases
Parasitic Diseases
medicine
Humans
Aged
Antiserum
Merozoites
Organisms
Infant
Biology and Life Sciences
Proteins
Tropical Diseases
biology.organism_classification
medicine.disease
Parasitic Protozoans
Malaria
030104 developmental biology
Immunoglobulin M
Immunoglobulin G
Immunologic Techniques
biology.protein
Parasitology
Apicomplexa
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 12, p e0243943 (2020)
Walker, M R, Knudsen, A S, Partey, F D, Bassi, M R, Frank, A M, Castberg, F C, Sarbah, E W, Ofori, M F, Hviid, L & Barfod, L 2020, ' Acquisition and decay of IgM and IgG responses to merozoite antigens after Plasmodium falciparum malaria in Ghanaian children ', PLoS ONE, vol. 15, no. 12, pp. e0243943 . https://doi.org/10.1371/journal.pone.0243943
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0243943
Popis: Developing a vaccine againstPlasmodium falciparummalaria has been challenging, primarily due to high levels of antigen polymorphism and a complex parasite lifecycle. Immunization with theP.falciparummerozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 has been shown to give rise to growth inhibitory and synergistic antisera. Therefore, these five merozoite proteins are considered to be promising candidates for a second-generation multivalent malaria vaccine. Nevertheless, little is known about IgG and IgM responses to these antigens in populations that are naturally exposed toP.falciparum. In this study, serum samples from clinically immune adults and malaria exposed children from Ghana were studied to compare levels of IgG and IgM specific for PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5. All five antigens were found to be specifically recognized by both IgM and IgG in serum from clinically immune adults and from children with malaria. Longitudinal analysis of the latter group showed an early, transient IgM response that was followed by IgG, which peaked 14 days after the initial diagnosis. IgG levels and parasitemia did not correlate, whereas parasitemia was weakly positively correlated with IgM levels. These findings show that IgG and IgM specific for merozoite antigens PfMSRP5, PfSERA9, PfRAMA, PfCyRPA and PfRH5 are high in children duringP.falciparummalaria, but that the IgM induction and decline occurs earlier in infection than that of IgG.
Databáze: OpenAIRE
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