Simultaneous Determination of Herbicides in Rice by QuEChERS and LC-MS/MS Using Matrix-Matched Calibration
| Autor: | Francisco C. Deschamps, Gilberto Abate, Maressa D. Dolzan, Gustavo Amadeu Micke, Melina Heller, Marco Grassi, Andrey Martinez Rebelo |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
liquid chromatography tandem mass spectrometry
Residue (complex analysis) multiresidue Chromatography ANVISA Electrospray ionization General Chemistry Imazapyr Quechers Tandem mass spectrometry High-performance liquid chromatography chemistry.chemical_compound herbicides chemistry Liquid chromatography–mass spectrometry Clomazone LC-MS/MS |
| Zdroj: | Journal of the Brazilian Chemical Society v.27 n.1 2016 Journal of the Brazilian Chemical Society Sociedade Brasileira de Química (SBQ) instacron:SBQ Journal of the Brazilian Chemical Society, Volume: 27, Issue: 1, Pages: 186-193, Published: JAN 2016 |
| Popis: | The main objective of the present work was to validate a chromatographic method to determine herbicides commonly applied in the irrigated rice farming. For this, matrix-matched calibration was employed along with the extraction and clean-up of the samples by quick, easy, cheap, effective, rugged and safe (QuEChERS) method and determination of the analytes by high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) with electrospray ionization in the positive mode. By this method, it was possible to achieve the ionization and detection of a total of 18 herbicides, with quantification of 12 of them. The method presented adequate precision and accuracy according to the European Commission and the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines for analytes in low concentrations. The limits of quantification ranged from 0.015 μg g-1 for oxadiazon to 0.165 μg g-1 for imazapyr. The method showed good linearity with R2> 0.99 and recovery values from 92 to 103%. The proposed protocol is adequate for monitoring bispyribac-sodium, cyclosulfamuron, cycloxydim, clomazone, ethoxysulfuron, fenoxaprop-p-ethyl, imazapic, imazapyr, imazethapyr, metsulfuron-methyl, oxadiazon and thiobencarb in rice grains in concentrations up to 109 times lower than the maximum residue limits established by the Brazilian Health Surveillance Agency (ANVISA) for these compounds in rice samples. |
| Databáze: | OpenAIRE |
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