Identification of early and late flowering time candidate genes in endodormant and ecodormant almond flower buds
| Autor: | Frank A Hoeberichts, Raquel Sánchez-Pérez, Federico Dicenta, Pedro Martínez-Gómez, Ángela S. Prudencio |
|---|---|
| Přispěvatelé: | Fundación Séneca, Ministerio de Economía y Competitividad (España), CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI) |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pollination Physiology Context (language use) Flowers Plant Science Biology medicine.disease_cause Tree breeding 01 natural sciences 03 medical and health sciences Plant Growth Regulators Gene Expression Regulation Plant Pollen medicine Climate change Dormancy Cultivar AcademicSubjects/SCI01210 Bud fungi DAM genes food and beverages Prunus dulcis Plant Breeding Horticulture 030104 developmental biology RNA-seq Fruit tree Research Paper 010606 plant biology & botany |
| Zdroj: | Tree Physiology Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Flower bud dormancy in temperate fruit tree species, such as almond [Prunus dulcis (Mill.) D.A. Webb], is a survival mechanism that ensures that flowering will occur under suitable weather conditions for successful flower development, pollination and fruit set. Dormancy is divided into three sequential phases: paradormancy, endodormancy and ecodormancy. During the winter, buds need cultivar-specific chilling requirements (CRs) to overcome endodormancy and heat requirements to activate the machinery to flower in the ecodormancy phase. One of the main factors that enables the transition from endodormancy to ecodormancy is transcriptome reprogramming. In this work, we therefore monitored three almond cultivars with different CRs and flowering times by RNA sequencing during the endodormancy release of flower buds and validated the data by quantitative real-Time PCR in two consecutive seasons. We were thus able to identify early and late flowering time candidate genes in endodormant and ecodormant almond flower buds associated with metabolic switches, transmembrane transport, cell wall remodeling, phytohormone signaling and pollen development. These candidate genes were indeed involved in the overcoming of the endodormancy in almond. This information may be used for the development of dormancy molecular markers, increasing the efficiency of temperate fruit tree breeding programs in a climate-change context. This study has been supported by Grants 19308/PI/14 and 19879/GERM/15 of the Seneca Foundation of the Region of Murcia (Spain) and the Almond Breeding project of the Spanish Ministry of Economy and Competitiveness. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Finally, RS-P is greatful for her RyC contract by the Ministery of Spanish Ministry of Economy and Competitiveness |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|