Cynomorium songaricum prevents bone resorption in ovariectomized rats through RANKL/RANK/TRAF6 mediated suppression of PI3K/AKT and NF-κB pathways
Autor: | Yanhong Dong, Jingjing Liu, Lingling Yang, Xueqin Ma, Bo Zhang, Qipeng Zhao |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Ovariectomy General Biochemistry Genetics and Molecular Biology Bone resorption Bone remodeling Rats Sprague-Dawley Phosphatidylinositol 3-Kinases 03 medical and health sciences 0302 clinical medicine Osteoprotegerin Internal medicine medicine Animals Bone Resorption General Pharmacology Toxicology and Pharmaceutics Protein kinase B TNF Receptor-Associated Factor 6 Bone mineral Cynomorium Receptor Activator of Nuclear Factor-kappa B biology Plant Extracts Chemistry RANK Ligand NF-kappa B Estradiol valerate General Medicine Rats 030104 developmental biology Endocrinology Gene Expression Regulation RANKL 030220 oncology & carcinogenesis Ovariectomized rat biology.protein Female Proto-Oncogene Proteins c-akt medicine.drug |
Zdroj: | Life Sciences. 209:140-148 |
ISSN: | 0024-3205 |
Popis: | Aim Cynomorium songaricum Rupr., an edible and important Traditional Chinese medicine has long been used in folk for treatment of kidney deficiency, was chosen to estimate the antiosteoporotic activity and underlying molecular mechanism on rats induced by ovariectomy (OVX). Main methods 9 of 45 rats were underwent bilateral laparotomy without removing the ovaries as sham group, remains were underwent bilateral ovariectomy and equally randomized into four groups: with vehicle (0.5% CMC-Na) as model group, estradiol valerate (1 mg/kg body weight/day) as positive control, with 100 and 300 mg/kg body weight/day of ethanol extracts of C. songaricum extract (CSE) as low and high dosage groups, respectively. Key findings After 12 weeks of continues orally intervention, the decreases of bone mineral density, bone mineral content, tissue mineral content, as well as the increases of bone trabecular separation and bone resorption markers were significantly reversed by CSE in the OVX rats, and in particular, a contradictory phenomenon on calcium and phosphorus contents was observed and elucidated. Mechanistically, the expressions of tumor-necrosis factor receptor-associated factor 6 (TRAF 6), nuclear factor kappa B (RANK) and its ligand (RANKL), as well as the nuclear factor kappa B (NF-κB), phosphoinositide 3‑kinase (PI3K) and protein kinase B (AKT) levels were significantly down-regulated by CSE intervention, whereas the osteoprotegerin (OPG) was significantly up-regulated by CSE as compared to the control. Significance Concisely, C. songaricum exhibited potential therapeutic effect on bone metabolism of ovariectomized rats, and this effect was possibly exerted by RANKL/RANK/TRAF6 mediated down-regulation of NF-κB and PI3K/AKT pathways. |
Databáze: | OpenAIRE |
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