Effect of D-penicillamine on rat lung elastin cross-linking during the perinatal period
| Autor: | Cetin Pekcetin, Gül Güner, Gülgün Oktay, Ataman Güre, Semra Koçtürk |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Offspring Clinical Biochemistry Biochemistry Desmosine chemistry.chemical_compound Pregnancy Internal medicine medicine Animals Isodesmosine Lung Chromatography High Pressure Liquid Skin biology Penicillamine Body Weight Ceruloplasmin Cell Biology General Medicine Organ Size medicine.disease Elasticity Elastin Rats medicine.anatomical_structure Endocrinology chemistry Animals Newborn Rheumatoid arthritis Prenatal Exposure Delayed Effects Immunology biology.protein Female Copper medicine.drug |
| Zdroj: | Cell biochemistry and function. 24(2) |
| ISSN: | 0263-6484 |
| Popis: | This study was designed to clarify the effects of D-penicillamine (DPA), a drug used for treatment of various pathological events, on lung elastin formation and maturation of the newborn in the perinatal period The investigation was conducted on 20 newborn rats bred from 40 female and six male rats. DPA doses 400 mg kg(-1) day(-1) and physiological saline were given intraperitoneally (i.p) to experimental and control goups. To assess newborn maturation, their body and lung weights were determined. Serum Cu levels were measured by atomic absorption spectroscopy and ceruloplasmin (Cp) activities were measured spectrophotometrically. Newborn lung tissue elastin, desmosine (DES) and isodesmosine (IDES) levels were measured by HPLC. The results showed that DPA treatment caused loss of skin elasticity and reduction in body and lung weight in newborns of the experimental group. The serum Cu levels and Cp activity were found to be significantly lower in both maternal and newborn of the experimental groups compared with the control group. The lung DES, IDES and elastin values of newborns in the experimental group were decreased compared with the control group. In conclusion, our results indicate that 400mg kg(-1) day(-1) DPA, a dose that is used in the treatment of Wilson's disease, rheumatoid arthritis and cystinuria, caused the retardation of newborn maturation, a decrease in DES-IDES cross-links and levels of lung elastin of offspring in the perinatal period. Another conclusion to be drawn from this study is that even low levels of Cu depletion due to DPA administration induces a change in cross-linking in lung elastin during the perinatal period. Copyright (c) 2005 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text